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Literature summary for 1.1.1.307 extracted from

  • Malla, S.; Gummadi, S.N.
    Simultaneous optimization of activity and stability of xylose reductase from D. nepalensis NCYC 3413 using statistical experimental design (2020), Protein Pept. Lett., 28, 489-500 .
    View publication on PubMed
Search for more literature for 1.1.1.307

Protein Variants

Protein Variants Comment Organism
additional information simultaneous optimization of xylose reductase activity and stability using statistical methods is effective as compared to optimisation of the parameters separately, effects of pH and temperature on the activity and stability of xylose reductase from Debaryomyces nepalensis NCYC 3413 are investigated by enzyme assays, and independent variables are optimised using surface response methodology. Enzyme activity and stability are optimised separately and concurrently to decipher the appropriate conditions, method comparisons, overview. Optimized conditions are pH 7.1 and 27°C with predicted responses of specific activity of 72.3 U/mg and half-life time of 566 min. The experimental values (specific activity 50.2 U/mg, half-life time 818 min) are on par with predicted values indicating the significance of the model Debaryomyces nepalensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADPH + H+ Debaryomyces nepalensis
-
 xylitol + NADP+
-
 r
D-xylose + NADPH + H+ Debaryomyces nepalensis NCYC 3413
-
 xylitol + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Debaryomyces nepalensis A0A0M4HL56
-
-
Debaryomyces nepalensis NCYC 3413 A0A0M4HL56
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50.2
-
 recombinant enzyme, pH 7.1, 27°C Debaryomyces nepalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADPH + H+
-
 Debaryomyces nepalensis xylitol + NADP+
-
 r
D-xylose + NADPH + H+
-
 Debaryomyces nepalensis NCYC 3413 xylitol + NADP+
-
 r

Synonyms

Synonyms Comment Organism
xylose reductase
-
 Debaryomyces nepalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 recombinant enzyme Debaryomyces nepalensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 recombinant enzyme Debaryomyces nepalensis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Debaryomyces nepalensis
NADPH
-
 Debaryomyces nepalensis