Cloned (Comment) | Organism |
---|---|
gene xyl1, recombinant expression of Xyl1 in Saccharomyces cerevisiae strain YPH499, co-expression with Aspergillus aculeatus beta-glucosidase 1 (AaBGL) and endogenous maltose transporter ScMAL11 | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae strain YPH499-SsXR-AaBGL expressing cytosolic Scheffersomyces stipitis xylose reductase (SsXR), along with Aspergillus aculeatus beta-glucosidase 1 (AaBGL) displayed on the cell surface. The simultaneous co-fermentation of cellobiose/xylose by this strain leads to an about 2.5fold increase in xylitol/xylose ratio compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XRScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose co-utilization. Method evaluation and optimization, overview | Scheffersomyces stipitis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Scheffersomyces stipitis | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Scheffersomyces stipitis | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Scheffersomyces stipitis NRRL Y-11545 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Scheffersomyces stipitis NBRC 10063 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Scheffersomyces stipitis ATCC 58785 | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | P31867 | - |
- |
Scheffersomyces stipitis ATCC 58785 | P31867 | - |
- |
Scheffersomyces stipitis NBRC 10063 | P31867 | - |
- |
Scheffersomyces stipitis NRRL Y-11545 | P31867 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Scheffersomyces stipitis | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Scheffersomyces stipitis NRRL Y-11545 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Scheffersomyces stipitis NBRC 10063 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | xylitol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SsXR | - |
Scheffersomyces stipitis |
XYL1 | - |
Scheffersomyces stipitis |
xylose reductase | - |
Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Scheffersomyces stipitis | |
NADPH | - |
Scheffersomyces stipitis |