Literature summary for 1.1.1.307 extracted from

Tani, T.; Taguchi, H.; Akamatsu, T.
Analysis of metabolisms and transports of xylitol using xylose- and xylitol-assimilating Saccharomyces cerevisiae (2017), J. Biosci. Bioeng., 123, 613-620 .

Search for more literature for 1.1.1.307

Cloned(Commentary)

Cloned (Comment)	Organism
gene xyl1, recombinant enzyme expression in Saccharomyces cerevisiae, different NAM34-4C (MATalpha) strain derivatives' genotypes and phenotypes, e.g. transformants of SCB38 with kanMX DNA or XM1, overview. XM1 is the DNA fragment carrying the genetic structure TDH3p-XYL1-TDH3t-TDH3p-XYL2-TDH3t-TDH3p-XKS1-TDH3t, including TDH3 promoter, TDH3 terminator, Scheffersomyces (Pichia) stipitis genes XYL1 (encoding NAD(P)H-dependent xylose reductase) and XYL2 (encoding NAD+-dependent xylitol dehydrogenase), and the endogenous XKS1 (encoding the Saccharomyces cerevisiae xylulokinase)	Scheffersomyces stipitis

Cloned (Comment)

Organism

gene xyl1, recombinant enzyme expression in Saccharomyces cerevisiae, different NAM34-4C (MATalpha) strain derivatives' genotypes and phenotypes, e.g. transformants of SCB38 with kanMX DNA or XM1, overview. XM1 is the DNA fragment carrying the genetic structure TDH3p-XYL1-TDH3t-TDH3p-XYL2-TDH3t-TDH3p-XKS1-TDH3t, including TDH3 promoter, TDH3 terminator, Scheffersomyces (Pichia) stipitis genes XYL1 (encoding NAD(P)H-dependent xylose reductase) and XYL2 (encoding NAD+-dependent xylitol dehydrogenase), and the endogenous XKS1 (encoding the Saccharomyces cerevisiae xylulokinase)

Scheffersomyces stipitis

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant enzyme expression in differently engineered Saccharomyces cerevisiae strains SCF201 and SCF202, aerobic or anaerobic batch fermentation cultures, measurement of xylitol consumption and ethanol production, analysis of culture condition effects, overview	Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
D-xylose + NADH + H+	Scheffersomyces stipitis	-	xylitol + NAD+	-	r
D-xylose + NADH + H+	Scheffersomyces stipitis NRRL Y-11545	-	xylitol + NAD+	-	r
D-xylose + NADH + H+	Scheffersomyces stipitis NBRC 10063	-	xylitol + NAD+	-	r
D-xylose + NADH + H+	Scheffersomyces stipitis ATCC 58785	-	xylitol + NAD+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis NRRL Y-11545	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis NBRC 10063	-	xylitol + NADP+	-	r
D-xylose + NADPH + H+	Scheffersomyces stipitis ATCC 58785	-	xylitol + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Scheffersomyces stipitis	P31867	-	-
Scheffersomyces stipitis ATCC 58785	P31867	-	-
Scheffersomyces stipitis NBRC 10063	P31867	-	-
Scheffersomyces stipitis NRRL Y-11545	P31867	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-xylose + NADH + H+	-	Scheffersomyces stipitis	xylitol + NAD+	-	r
D-xylose + NADH + H+	-	Scheffersomyces stipitis NRRL Y-11545	xylitol + NAD+	-	r
D-xylose + NADH + H+	-	Scheffersomyces stipitis NBRC 10063	xylitol + NAD+	-	r
D-xylose + NADH + H+	-	Scheffersomyces stipitis ATCC 58785	xylitol + NAD+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis NRRL Y-11545	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis NBRC 10063	xylitol + NADP+	-	r
D-xylose + NADPH + H+	-	Scheffersomyces stipitis ATCC 58785	xylitol + NADP+	-	r

Synonyms

Synonyms	Comment	Organism
NAD(P)H-dependent xylose reductase	-	Scheffersomyces stipitis
XYL1	-	Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	in vivo assay at	Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	in vivo assay at	Scheffersomyces stipitis

Cofactor

Cofactor	Comment	Organism
NAD+	-	Scheffersomyces stipitis
NADH	-	Scheffersomyces stipitis
NADP+	-	Scheffersomyces stipitis
NADPH	-	Scheffersomyces stipitis

General Information

General Information	Comment	Organism
metabolism	in anaerobic culture, NAD+ generated in the NAD(P)H-dependent xylose reductase reaction is likely needed in the NAD+-dependent xylitol dehydrogenase reaction, whereas in aerobic culture, the NAD+ generated by oxidation of NADH in the mitochondria is required in the xylitol dehydrogenase reaction, analysis of the relationship between NAD(P)+/NAD(P)H redox balances and metabolisms of xylose or xylitol as carbon sources in aerobic and anaerobic batch cultures of recombinant Saccharomyces cerevisiae in a complex medium containing 20 g/l xylose or 20 g/l xylitol at pH 5.0 and 30°C. Addition of acetaldehyde (an effective oxidizer of NADH) increases the xylitol consumption by the anaerobically cultured strain. Gal2 and Fps1 transport xylitol both inward and outward and play a role in xylitol consumption importing xylitol into the cytosol and exporting it from the cells	Scheffersomyces stipitis