Cloned (Comment) | Organism |
---|---|
gene budC, functional recombinant expression of NADH-dependent Kp-BudC from plasmid pKM3 in Escherichia coli wild-type strain BW25113 and in mutant strain JCL166 (BW25113/F' [traD36, proABþ, lacIqZ DELTAM15 (Tet)] JCL16 DELTAadhE DELTAldhA DELTAfrdBC) | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance, overview. Anaerobic growth rescue with feeding of acetoin of the deletion mutant strain JCL166 transformed with the designated plasmid carrying the Kp-BudC, Cb-Adh, or the Ec-GldA. substrate-decoupled selection platform based on anaerobic redox balance | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADH + H+ | Klebsiella pneumoniae | - |
(S)-acetoin + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q48436 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADH + H+ | - |
Klebsiella pneumoniae | (S)-acetoin + NAD+ | - |
? | |
diacetyl + NADH + H+ | stereospecific reaction | Klebsiella pneumoniae | (S)-acetoin + NAD+ | - |
? | |
additional information | the enzyme BudC also catalyzes the reduction of acetoin by NADH, cf. EC 1.1.1.76 | Klebsiella pneumoniae | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
budC | - |
Klebsiella pneumoniae |
DAR | - |
Klebsiella pneumoniae |
diacetyl reductase | - |
Klebsiella pneumoniae |
Kp-BudC | - |
Klebsiella pneumoniae |
Kpdar | - |
Klebsiella pneumoniae |
NADH-dependent Kp-BudC | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Klebsiella pneumoniae |