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Literature summary for 1.1.1.300 extracted from
- Evidence that proteosome inhibitors and chemical chaperones can rescue the activity of retinol dehydrogenase 12 mutant T49M (2011), Chem. Biol. Interact., 191, 55-59.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in HEK-293 cell microsomes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I51N | site-directed mutagenesis, the catalytically active I51N variant of RDH12 undergoes accelerated degradation through the ubiquitin-proteosome system, which results in reduced level of the protein in the cell. The RDH12 mutant has lost its retinaldehyde reductase activity. Inhibitors of proteosome activity, e.g. MG132, or dimethyl sulfoxide can partially restore the activity | Homo sapiens |
| T49M | site-directed mutagenesis, the catalytically active T49M variant of RDH12 undergoes accelerated degradation through the ubiquitin-proteosome system, which results in reduced level of the protein in the cell.The RDH12 mutant has lost its retinaldehyde reductase activity. Inhibitors of proteosome activity, e.g. MG132, or dimethyl sulfoxide can partially restore the activity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-retinal + NADPH + H+ | Homo sapiens | - |
all-trans-retinol + NADP+ | - |
r |  |
| all-trans-retinol + NADP+ | Homo sapiens | - |
all-trans-retinal + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from HEK-293 cell microsomes | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-retinal + NADPH + H+ | - |
Homo sapiens | all-trans-retinol + NADP+ | - |
r | |
| all-trans-retinol + NADP+ | - |
Homo sapiens | all-trans-retinal + NADPH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RDH12 | - |
Homo sapiens |
| retinaldehyde reductase | - |
Homo sapiens |
| retinol dehydrogenase 12 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | dependent on | Homo sapiens | |
| NADPH | - |
Homo sapiens | |
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