Literature summary for 1.1.1.300 extracted from

Belyaeva, O.V.; Korkina, O.V.; Stetsenko, A.V.; Kedishvili, N.Y.
Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase/reductase with a retinaldehyde reductase activity (2008), FEBS J., 275, 138-147.

Search for more literature for 1.1.1.300

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Sf9 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,2-diheptanoyl-sn-glycero-3-phosphocholine	substitution of the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine for Tween-20 results in complete inactivation of the enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	NADPH	-	Homo sapiens
0.0032	-	all-trans-retinal	-	Homo sapiens
0.004	-	all-trans-retinol	Km-value for all-trans-retinol is similar to that for retinal however, the rate of retinol oxidation by RDH13 is extremely low	Homo sapiens
6	-	NADH	pH and temperature not specified in the publication. The apparent Km value for NADH is three orders of magnitude higher than that for NADPH	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial membrane	RDH13 is localized on the outer side of the inner mitochondrial membrane	Homo sapiens	31966	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
all-trans-retinal + NADPH + H+	Homo sapiens	greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene	all-trans-retinol + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8NBN7	-	-

Oxidation Stability

Oxidation Stability	Organism
requires reducing conditions to stay active	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	high expression level	Homo sapiens	-
kidney	high expression level	Homo sapiens	-
lung	high expression level	Homo sapiens	-
ovary	moderate expression level	Homo sapiens	-
prostate	moderate expression level	Homo sapiens	-
testis	moderate expression level	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
all-trans-retinal + NADPH + H+	greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene	Homo sapiens	all-trans-retinol + NADP+	-	?
all-trans-retinal + NADPH + H+	prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH	Homo sapiens	all-trans-retinol + NADP+	-	?
additional information	no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids	Homo sapiens	?	-	?
retinal + NADH + H+	-	Homo sapiens	retinol + NAD+	-	r

Synonyms

Synonyms	Comment	Organism
RDH13	-	Homo sapiens
retinol dehydrogenase 13	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	partially inactivated after 20 min	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADH	prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH	Homo sapiens
NADPH	prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)