Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE) cells | Ignicoccus islandicus |
Crystallization (Comment) | Organism |
---|---|
apoenzyme, sitting drop vapor diffusion method, using 10% (w/v) PEG 6000 and 100 mM EPES at pH 7.0 | Ignicoccus islandicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | - |
Ignicoccus islandicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
oxaloacetate | at pH 7.0 and 70°C | Ignicoccus islandicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
124000 | - |
size exclusion chromatography-multi angle laser light scattering | Ignicoccus islandicus |
133000 | - |
calculated from amino acid sequence | Ignicoccus islandicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | Ignicoccus islandicus | - |
(S)-malate + NAD+ | - |
? | |
oxaloacetate + NADPH + H+ | Ignicoccus islandicus | - |
(S)-malate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ignicoccus islandicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and Superpose 12 gel filtration | Ignicoccus islandicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Ignicoccus islandicus | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | the enzyme strongly prefers oxaloacetate by three orders of magnitude compared to pyruvate | Ignicoccus islandicus | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADPH + H+ | - |
Ignicoccus islandicus | (S)-malate + NADP+ | - |
? | |
pyruvate + NADH + H+ | weak catalytic efficiency with pyruvate | Ignicoccus islandicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Ignicoccus islandicus |
Synonyms | Comment | Organism |
---|---|---|
LDH-like MalDH | - |
Ignicoccus islandicus |
NAD(P)-dependent malate dehydrogenase | - |
Ignicoccus islandicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
pyruvate | at pH 6.0 and 70°C | Ignicoccus islandicus | |
444 | - |
oxaloacetate | at pH 7.0 and 70°C | Ignicoccus islandicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ignicoccus islandicus | |
NADP+ | - |
Ignicoccus islandicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
oxaloacetate | at pH 7.0 and 70°C | Ignicoccus islandicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
oxaloacetate | at pH 7.0 and 70°C | Ignicoccus islandicus |