Cloned (Comment) | Organism |
---|---|
gene mcr, recombinant expression of MCR enzyme mutant in Escherichia coli strain CE43(DE3), coexpression with malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris, native transhydrogenase (PntAB), and NAD kinase (YfjB) | Chloroflexus aurantiacus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of 3-hydroxypropionate using a novel malonyl-CoA-mediated biosynthetic pathway in genetically engineered Escherichia coli strain. Heterologously coexpressing the mutant of malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus and malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris in the Escherichia coli C43 (DE3) strain. To further enhance the production of 3-HP, native transhydrogenase (PntAB) and NAD kinase (YfjB) genes are expressed to increase the NADPH supply in Escherichia coli. The final genetically modified strain SGN78 shows a significant improvement in malonate utilization and produced 1.20 g/l of 3-HP in the flask culture. Identification of suitable malonate transporters in Rhodobacter capsulatus and Sinorhizobium meliloti, and coexpression of transporter MatB in Escherichia coli. The enzyme activity increases when the N-terminal and C-terminal regions of MCR are separated by fusing a flexible linker (GGGGS) between the two enzymatic units. Optimization of fermentation conditions and improvement of NADPH supply increase 3-HP production rate | Chloroflexus aurantiacus |
N940V/K1106W/S1114R | site-directed mutagenesis, mutant N940V/K1106W/S1114R improves the catalytic efficiency by 14.2fold over the wild-type | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADPH + H+ | Chloroflexus aurantiacus | - |
3-hydroxypropanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q6QQP7 | bifunctional enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADPH + H+ | - |
Chloroflexus aurantiacus | 3-hydroxypropanoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA reductase | UniProt | Chloroflexus aurantiacus |
MCR | - |
Chloroflexus aurantiacus |
More | see also EC 1.2.1.75 | Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chloroflexus aurantiacus |