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Literature summary for 1.1.1.292 extracted from

  • Nasher, F.; Foerster, S.; Yildirim, E.; Grandgirard, D.; Leib, S.; Heller, M.; Hathaway, L.
    Foreign peptide triggers boost in pneumococcal metabolism and growth (2018), BMC Microbiol., 18, 23 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.292

Activating Compound

Activating Compound Comment Organism Structure
additional information peptide FPPQSV is absolutely required for induction of the enzyme via AliB-like ORF 2, no activity without the peptide Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
gene afr, quantitative real-time RT-PCR enzyme expression analysis Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-anhydro-D-fructose + NADPH + H+ Streptococcus pneumoniae
-
 1,5-anhydro-D-mannitol + NADP+
-
 r
1,5-anhydro-D-fructose + NADPH + H+ Streptococcus pneumoniae 110.58
-
 1,5-anhydro-D-mannitol + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae A0A064C3N0
-
-
Streptococcus pneumoniae 110.58 A0A064C3N0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADPH + H+
-
 Streptococcus pneumoniae 1,5-anhydro-D-mannitol + NADP+
-
 r
1,5-anhydro-D-fructose + NADPH + H+
-
 Streptococcus pneumoniae 110.58 1,5-anhydro-D-mannitol + NADP+
-
 r

Synonyms

Synonyms Comment Organism
AFR
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Streptococcus pneumoniae
NADPH
-
 Streptococcus pneumoniae

Expression

Organism Comment Expression
Streptococcus pneumoniae peptide FPPQSV induces the expression of 1,5-anhydro-D-fructose (AF). The peptide is found in Prevotella species, resulting in enhanced colonization, proteomic analysis is performed by LC-MS/MS on wild-type strain 110.58 and mutant DELTAORF 2 in the presence and absence of AliB-like ORF 2 peptide ligand FPPQSV up

General Information

General Information Comment Organism
physiological function nonencapsulated Streptococcus pneumoniae bacteria are successful colonizers of the human nasopharynx and often possess genes aliB-like ORF 1 and 2 in place of capsule genes. AliB-like ORF 2 binds peptide FPPQSV, found in Prevotella species, resulting in enhanced colonization. 1,5-anhydro-D-fructose reductase is a metabolic enzyme of an alternative starch and glycogen degrading pathway found in many organisms, in both transcriptomic and proteomic data. The peptide increases expression of genes involved in release of host carbohydrates, carbohydrate uptake and carbohydrate metabolism. AliB-like ORF 2 confers an advantage in colonization by enhancing carbohydrate metabolism resulting in a boost in growth. This may explain the widespread presence of aliB-like ORF 2 in the nonencapsulated pneumococcal population in the human nasopharynx. Peptide FPPQSV is absolutely required for induction of enzyme 1,5-anhydro-D-fructose reductase (AFR) via AliB-like ORF 2, no activity without the peptide Streptococcus pneumoniae