Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme SlGSNOR knockout by gene silencing with RNAi using Agrobacterium tumefaciens transfection method. The accumulation of SlGSNOR1 transcripts is tightly regulated in tomato MicroTom and a significant reduction in its expression leads to lethality, as SlGSNOR-RNAi lines with greater than about 60% reduction in SlGSNOR1 expression are not viable. Both SlGSNOR-RNAi and SlGSNOR-overexpressing(OE) lines convey significant effects on the overall development of tomato plants, ranging from seed germination to fruiting and net yield per plant. Reduced GSNOR expression in SlGSNOR-RNAi lines drastically affects seed development and reduces the number of seeds produced in the fruits of the resulting transgenic plants, phenotype, overview. SlGSNOR-RNAi plants also show faster germination as compared with wild-type and SlGSNOR-OE plants on either MS medium or soil, and show the appearance of fresh green tissues at least 1 d before the wild-type and overexpressing plants | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADPH + H+ | Solanum lycopersicum | - |
GSSG + ammonia + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | D2Y3F4 | var. MicroTom | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADPH + H+ | - |
Solanum lycopersicum | GSSG + ammonia + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
GSNOR | - |
Solanum lycopersicum |
S-nitrosoglutathione reductase | - |
Solanum lycopersicum |
SlGSNOR | - |
Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of GSNOR function impacts tomato (Solanum lycopersicum. L) fruit development. Thus, reduction of GSNOR expression through RNAi modulated both fruit formation and yield, establishing another function for GSNOR. Further, depletion of Solanum lycopersicum GSNOR (SlGSNOR) additionally impacted a number of other developmental processes, including seed development, which also has not been previously linked with GSNOR activity. Depletion of GSNOR function does not influence root development in tomato. Reduction of GSNOR transcript abundance compromises plant immunity. Overexpression of SlGSNOR promotes resistance to bacterial pathogens, such as Pseudomonas syringae pv. tomato DC3000 (PstDC3000) | Solanum lycopersicum |
physiological function | nitric oxide (NO) is emerging as a key signalling molecule in plants. The chief mechanism for the transfer of NO bioactivity is thought to be S-nitrosylation, the addition of an NO moiety to a protein cysteine thiol to form an S-nitrosothiol (SNO). The enzyme S-nitrosoglutathione reductase (GSNOR) indirectly controls the total levels of cellular S-nitrosylation, by depleting S-nitrosoglutathione (GSNO), the major cellular NO donor. SlGSNOR regulates seed devlopment, fruit production, and flower development, overview | Solanum lycopersicum |