Literature summary for 1.1.1.281 extracted from

Chin, Y.W.; Park, J.B.; Park, Y.C.; Kim, K.H.; Seo, J.H.
Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose (2013), Bioprocess Biosyst. Eng., 36, 749-756.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene rmd, constitutive recombinant overexpression in Spodoptera frugiperda Sf9 cells or in Trichoplusia ni High Five cells under the transcriptional control of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) immediate early 1 (ie1) promoter and via baculovirus transfection. Rmd to blocks the production of GDP-L-fucose and glycoprotein fucosylation because GDP-L-fucose is required as the donor substrate for this process	Pseudomonas aeruginosa

Cloned (Comment)

Organism

gene rmd, constitutive recombinant overexpression in Spodoptera frugiperda Sf9 cells or in Trichoplusia ni High Five cells under the transcriptional control of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) immediate early 1 (ie1) promoter and via baculovirus transfection. Rmd to blocks the production of GDP-L-fucose and glycoprotein fucosylation because GDP-L-fucose is required as the donor substrate for this process

Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
additional information	GDP-L-fucose biosynthesis in eukaryotic cells is reduced by expression of bacterial Rmd, which converts GDP-4-dehydro-6-deoxy-D-mannose to GDP-D-rhamnose, assessing a vector-based glycoengineering strategy for blocking glycoprotein fucosylation in insect cells, overview. N-glycan profiling by MALDI-TOF mass spectrometry	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+	Pseudomonas aeruginosa	-	GDP-D-rhamnose + NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HTB6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+	-	Pseudomonas aeruginosa	GDP-D-rhamnose + NAD(P)+	-	?

Synonyms

Synonyms	Comment	Organism
GDP-6-deoxy-D-mannose reductase	-	Pseudomonas aeruginosa
RMD	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)H	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
metabolism	the enzyme consumes the immediate precursor to GDP-L-fucose to produce GDP-D-rhamnose, which might be a dead-end product in recombinant insect cells	Pseudomonas aeruginosa