Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme from Pediococcus pentosaceus can be used for production of 3-phenyllactic acid (2-hydroxy-3-phenylpropanoic acid, PLA), an antimicrobial compound with broad spectrum activity against both bacteria and fungi, optimization by coexpression of Ogataea parapolymorpha formate dehydrogenase, EC 1.2.1.2, for NADH regeneration | Pediococcus pentosaceus |
Cloned (Comment) | Organism |
---|---|
co-overexpression of Pediococcus pentosaceus D-lactate dehydrogenase and Ogataea parapolymorpha FDH as His6-tagged enzymes in Escherichia coli strain BL21(DE3) | Pediococcus pentosaceus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzymatic production of D-3-phenyllactic acid by recombinant Pediococcus pentosaceus D-lactate dehydrogenase with NADH regeneration by recombinant Ogataea parapolymorpha formate dehydrogenase, EC 1.2.1.2, at pH 6.0, 50°C | Pediococcus pentosaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | inhibits D-3-phenyllactate biotransformation | Pediococcus pentosaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-phenylpyruvate + NADH + H+ | Pediococcus pentosaceus | - |
D-3-phenyllactate + NAD+ | - |
r | |
additional information | Pediococcus pentosaceus | enzymatic production of D-3-phenyllactic acid by recombinant Pediococcus pentosaceus D-lactate dehydrogenase with NADH regeneration by recombinant Ogataea parapolymorpha formate dehydrogenase, EC 1.2.1.2 | ? | - |
? | |
pyruvate + NADH + H+ | Pediococcus pentosaceus | - |
(R)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus pentosaceus | Q9AKS9 | - |
- |
Purification (Comment) | Organism |
---|---|
copurification of recombinant His6-tagged D-lactate dehydrogenase and His6-tagged Ogataea parapolymorpha FDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pediococcus pentosaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-phenylpyruvate + NADH + H+ | - |
Pediococcus pentosaceus | D-3-phenyllactate + NAD+ | - |
r | |
D-3-phenylpyruvate + NADH + H+ | - |
Pediococcus pentosaceus | D-3-phenyllactate + NAD+ | i.e. 2-hydroxy-3-phenylpropanoic acid | r | |
additional information | enzymatic production of D-3-phenyllactic acid by recombinant Pediococcus pentosaceus D-lactate dehydrogenase with NADH regeneration by recombinant Ogataea parapolymorpha formate dehydrogenase, EC 1.2.1.2 | Pediococcus pentosaceus | ? | - |
? | |
pyruvate + NADH + H+ | - |
Pediococcus pentosaceus | (R)-lactate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Pediococcus pentosaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pediococcus pentosaceus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | activity range, profile overview | Pediococcus pentosaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pediococcus pentosaceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range, profile overview | Pediococcus pentosaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pediococcus pentosaceus | |
NADH | - |
Pediococcus pentosaceus |