Cloned (Comment) | Organism |
---|---|
gene ldhD, quantitative enzyme realtime PCR expression analysis during ferentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactobacillus delbrueckii subsp. bulgaricus |
gene ldhD, quantitative enzyme realtime PCR expression analysis during fermentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Weizmannia coagulans |
gene ldhD, quantitative enzyme realtime PCR expression analysis during fermentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactiplantibacillus plantarum subsp. plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus | |
2.7 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
5.9 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
9.54 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Weizmannia coagulans | the enzyme shows very low in vivo activity | ? | - |
? | |
additional information | Weizmannia coagulans 2-6 | the enzyme shows very low in vivo activity | ? | - |
? | |
pyruvate + NADH + H+ | Lactobacillus delbrueckii subsp. bulgaricus | - |
(R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Weizmannia coagulans | - |
(R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Lactiplantibacillus plantarum subsp. plantarum | - |
(R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | - |
(R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | - |
(R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Weizmannia coagulans 2-6 | - |
(R)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum subsp. plantarum | C0LJH4 | - |
- |
Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | C0LJH4 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus | P26297 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | P26297 | - |
- |
Weizmannia coagulans | F8RPR8 | - |
- |
Weizmannia coagulans 2-6 | F8RPR8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus delbrueckii subsp. bulgaricus |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Weizmannia coagulans |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactiplantibacillus plantarum subsp. plantarum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain 2-6 is an L-lactic acid producer, it is a homofermentative producer of L-lactic acid with an optical purity of 99% | Weizmannia coagulans | - |
additional information | strain DSM 20081 is a D-lactic acid producer producing D-lactic acid at an optical purity of 98% | Lactobacillus delbrueckii subsp. bulgaricus | - |
additional information | strain DSM 20174 is a DL-lactic acid producer, 1:1 ratio of L-lactic acid to D-lactic acid | Lactiplantibacillus plantarum subsp. plantarum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
crude enzyme, pyruvate reduction in vivo activity is below detection limit | Weizmannia coagulans |
2.51 | - |
crude enzyme, pyruvate reduction in vivo activity, pH 6.5, 30°C | Lactiplantibacillus plantarum subsp. plantarum |
8.3 | - |
crude enzyme, pyruvate reduction in vivo activity, pH 6.5, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows very low in vivo activity | Weizmannia coagulans | ? | - |
? | |
additional information | the enzyme shows very low in vivo activity | Weizmannia coagulans 2-6 | ? | - |
? | |
pyruvate + NADH + H+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Weizmannia coagulans | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Lactiplantibacillus plantarum subsp. plantarum | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Weizmannia coagulans 2-6 | (R)-lactate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-nLDH | - |
Lactobacillus delbrueckii subsp. bulgaricus |
D-nLDH | - |
Weizmannia coagulans |
D-nLDH | - |
Lactiplantibacillus plantarum subsp. plantarum |
ldhd | - |
Lactobacillus delbrueckii subsp. bulgaricus |
ldhd | - |
Weizmannia coagulans |
ldhd | - |
Lactiplantibacillus plantarum subsp. plantarum |
NAD-dependent D-lactate dehydrogenase | - |
Lactobacillus delbrueckii subsp. bulgaricus |
NAD-dependent D-lactate dehydrogenase | - |
Weizmannia coagulans |
NAD-dependent D-lactate dehydrogenase | - |
Lactiplantibacillus plantarum subsp. plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactiplantibacillus plantarum subsp. plantarum |
35 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus |
35 | - |
- |
Weizmannia coagulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
11.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
91.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
235.5 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Lactobacillus delbrueckii subsp. bulgaricus |
6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Weizmannia coagulans |
6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Lactiplantibacillus plantarum subsp. plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
NAD+ | - |
Weizmannia coagulans | |
NAD+ | - |
Lactiplantibacillus plantarum subsp. plantarum | |
NADH | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
NADH | - |
Weizmannia coagulans | |
NADH | - |
Lactiplantibacillus plantarum subsp. plantarum |
General Information | Comment | Organism |
---|---|---|
additional information | ldhD transcription levels are higher than those of ldhL, EC 1.1.1.27 | Lactobacillus delbrueckii subsp. bulgaricus |
additional information | ldhD transcription levels are higher than those of ldhL, EC 1.1.1.27 | Lactiplantibacillus plantarum subsp. plantarum |
additional information | ldhL, EC 1.1.1.27, transcription levels are higher than those of ldhD | Weizmannia coagulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
1.9 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
34 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
7900 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus |