Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-bisphosphate | required | Fusobacterium nucleatum subsp. nucleatum | |
fructose 1,6-bisphosphate | required | Pseudomonas aeruginosa | |
additional information | no activation of the enzyme by fructose 1,6-bisphosphate and Mg2+ | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene ecd_01352, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Escherichia coli |
gene fn0511, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Fusobacterium nucleatum subsp. nucleatum |
gene pa0927, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyoxylate | - |
Pseudomonas aeruginosa | |
Hydroxypyruvate | - |
Pseudomonas aeruginosa | |
oxaloacetate | - |
Escherichia coli | |
oxaloacetate | - |
Fusobacterium nucleatum subsp. nucleatum | |
oxamate | constant inhibition at pH 7.0 and pH 8.0 | Escherichia coli | |
oxamate | inhibits the catalytic reaction of FNLDH competitively with pyruvate, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 | Fusobacterium nucleatum subsp. nucleatum | |
oxamate | inhibits the catalytic reaction of PALDH in a mixed--type manner at pH 7.0, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 | Pseudomonas aeruginosa | |
phenylpyruvate | - |
Fusobacterium nucleatum subsp. nucleatum | |
pyruvate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5 | Pseudomonas aeruginosa | |
additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 | Fusobacterium nucleatum subsp. nucleatum | |
additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 | Escherichia coli | |
0.019 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.022 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
0.028 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
0.031 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
0.035 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ | Pseudomonas aeruginosa | |
0.036 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
0.055 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
0.088 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
0.09 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
0.092 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
0.1 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.31 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
0.34 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
0.36 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
0.64 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.68 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
0.85 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
1.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.7 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.7 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
2.5 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
2.5 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
2.6 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
2.6 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
3 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
3.5 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
3.8 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
4 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
5.8 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
6.5 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
6.8 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
7.1 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
7.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
7.6 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
11 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
12 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
12 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
18 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
18 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
20 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
30 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
54 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Fusobacterium nucleatum subsp. nucleatum | |
Mg2+ | activates | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
- |
Pseudomonas aeruginosa |
38500 | - |
- |
Escherichia coli |
40000 | - |
4 * 40000, about, sequence calculation | Fusobacterium nucleatum subsp. nucleatum |
120000 | - |
recombinant His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
150000 | - |
recombinant His-tagged enzyme, peak 2, gel filtration | Escherichia coli |
160000 | - |
recombinant His-tagged enzyme, gel filtration | Fusobacterium nucleatum subsp. nucleatum |
330000 | - |
recombinant His-tagged enzyme, peak 1, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Fusobacterium nucleatum subsp. nucleatum | - |
pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | Escherichia coli | - |
pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | Pseudomonas aeruginosa | - |
(R)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A140N893 | - |
- |
Fusobacterium nucleatum subsp. nucleatum | Q8RG11 | strain JCM 8532 | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | Q8RG11 | strain JCM 8532 | - |
Pseudomonas aeruginosa | Q9I530 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Fusobacterium nucleatum subsp. nucleatum |
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Pseudomonas aeruginosa |
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Fusobacterium nucleatum subsp. nucleatum | |
(R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Pseudomonas aeruginosa | |
(R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Fusobacterium nucleatum subsp. nucleatum | pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | - |
Escherichia coli | pyruvate + NADH + H+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Escherichia coli | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxycaproate + NAD+ | - |
r | |
2-oxocaproate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxycaproate + NAD+ | - |
r | |
2-oxocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | 2-hydroxycaproate + NAD+ | - |
r | |
2-oxoisocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyisocaproate + NAD+ | - |
r | |
2-oxoisocaproate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxyisocaproate + NAD+ | - |
r | |
2-oxoisovalerate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyisovalerate + NAD+ | - |
r | |
2-oxovalerate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyvalerate + NAD+ | - |
r | |
2-oxovalerate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxyvalerate + NAD+ | - |
r | |
glyoxylate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | hydroxyacetate + NAD+ | - |
r | |
glyoxylate + NADH + H+ | - |
Pseudomonas aeruginosa | hydroxyacetate + NAD+ | - |
r | |
glyoxylate + NADH + H+ | - |
Escherichia coli | hydroxyacetate + NAD+ | - |
r | |
hydroxypyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH + H+ | - |
Escherichia coli | glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | glycerate + NAD+ | - |
r | |
additional information | the enzyme ECLDH exhibits high substrate specificity toward pyruvate. No or poor activity with 2-oxovalerate, 2-oxoisovalerate, 2-oxocaproate, 2-oxoisocaproate, phenylpyruvate, and hydroxyphenylpyruvate | Escherichia coli | ? | - |
? | |
additional information | the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Fusobacterium nucleatum subsp. nucleatum | ? | - |
? | |
additional information | the enzyme PALDH exhibits high substrate specificity toward pyruvate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Pseudomonas aeruginosa | malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Escherichia coli | malate + NAD+ | - |
r | |
phenylpyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | D-phenyllactate + NAD+ | - |
r | |
phenylpyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | D-phenyllactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Escherichia coli | (R)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | (R)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 38500, about, sequence calculation, the apparently octameric form of ECLDH is likely an artificial product due to the overexpression, and exhibits slightly but significantly lower specific activity than the tetrameric form | Escherichia coli |
homotetramer | 4 * 40000, about, sequence calculation | Fusobacterium nucleatum subsp. nucleatum |
homotetramer | 4 * 38500, about, sequence calculation | Escherichia coli |
homotrimer | 4 * 37800, about, sequence calculation | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Fusobacterium nucleatum subsp. nucleatum |
D-LDH | - |
Pseudomonas aeruginosa |
D-LDH | - |
Escherichia coli |
ECBD_2243 | - |
Escherichia coli |
ECLDH | - |
Escherichia coli |
FN0511 | - |
Fusobacterium nucleatum subsp. nucleatum |
FNLDH | - |
Fusobacterium nucleatum subsp. nucleatum |
PA0927 | - |
Pseudomonas aeruginosa |
PALDH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Fusobacterium nucleatum subsp. nucleatum |
30 | - |
assay at | Pseudomonas aeruginosa |
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
purified recombinant His-tagged enzyme, pH 8.0, stable up to | Fusobacterium nucleatum subsp. nucleatum |
49 | - |
purified recombinant His-tagged enzyme, pH 8.0, stable up to | Escherichia coli |
57 | - |
purified recombinant His-tagged enzyme, pH 5.0, stable up to | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
18 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
31 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
39 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
50 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
69 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
80 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
80 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
80 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
80 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
87 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
90 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
98 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
100 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
100 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
100 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
110 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
120 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
120 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
140 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
140 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
160 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
160 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
200 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
230 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
240 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
240 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
270 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ | Pseudomonas aeruginosa | |
290 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
310 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
370 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
380 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
400 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
400 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
410 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
410 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
420 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
640 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
690 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
710 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
730 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
880 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1100 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Fusobacterium nucleatum subsp. nucleatum |
7 | 8 | assay at | Pseudomonas aeruginosa |
7 | 8 | assay at | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Pseudomonas aeruginosa |
5 | 10 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Escherichia coli |
5 | 8.5 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Fusobacterium nucleatum subsp. nucleatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Fusobacterium nucleatum subsp. nucleatum | |
NAD+ | - |
Pseudomonas aeruginosa | |
NAD+ | - |
Escherichia coli | |
NADH | - |
Fusobacterium nucleatum subsp. nucleatum | |
NADH | - |
Pseudomonas aeruginosa | |
NADH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Fusobacterium nucleatum subsp. nucleatum | |
additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
1.8 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
5 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
19 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
20 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
23 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
44 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
140 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum |
General Information | Comment | Organism |
---|---|---|
evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Fusobacterium nucleatum subsp. nucleatum |
evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Pseudomonas aeruginosa |
evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Escherichia coli |
metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Fusobacterium nucleatum subsp. nucleatum |
metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Pseudomonas aeruginosa |
metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
2.6 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
3.2 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
4.2 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
5.3 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
8.7 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
11 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
18 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
21 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
27 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
31 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
35 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
35 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
50 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
51 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
53 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
60 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
61 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
65 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
87 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
130 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
140 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
150 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
160 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
200 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
200 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
230 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
230 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
280 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
440 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
820 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
2500 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
2800 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
3700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
4000 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
4400 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
7800 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
8000 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
8100 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
12000 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
17000 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa |