Cloned (Comment) | Organism |
---|---|
expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pediococcus acidilactici |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits 35% at 1 mM | Pediococcus acidilactici | |
Fe2+ | inhibits 25% at 1 mM | Pediococcus acidilactici |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
2.9 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates by 25% at 1 mM | Pediococcus acidilactici | |
additional information | poor effects by 1 mM Ca2+, Zn2+, Mg2+, Ba2+, and Co2+ | Pediococcus acidilactici |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Pediococcus acidilactici | - |
pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | Pediococcus acidilactici DSM 20284 | - |
pyruvate + NADH + H+ | - |
r | |
phenylpyruvate + NADH + H+ | Pediococcus acidilactici | - |
(R)-3-phenyllactate + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | Pediococcus acidilactici DSM 20284 | - |
(R)-3-phenyllactate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus acidilactici | - |
- |
- |
Pediococcus acidilactici DSM 20284 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pediococcus acidilactici |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
purified recombinant enzyme, substrate phenylpyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
422 | - |
purified recombinant enzyme, substrate pyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Pediococcus acidilactici | pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | - |
Pediococcus acidilactici DSM 20284 | pyruvate + NADH + H+ | - |
r | |
phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici | (R)-3-phenyllactate + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | (R)-3-phenyllactate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Pediococcus acidilactici |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pediococcus acidilactici |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile, overview | Pediococcus acidilactici |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | the enzyme is significantly stable below 30°C, but the activity decreases significantly above 40°C | Pediococcus acidilactici |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
305 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pediococcus acidilactici |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | activity range, profile, overview | Pediococcus acidilactici |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pediococcus acidilactici | |
NADH | - |
Pediococcus acidilactici |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
105 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
3157 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |