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Literature summary for 1.1.1.27 extracted from

  • Skory, C.D.; Ibrahim, A.S.
    Native and modified lactate dehydrogenase expression in a fumaric acid producing isolate Rhizopus oryzae 99-880 (2007), Curr. Genet., 52, 23-33.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ldhB, from strain NRRL 395 and 99-880, expression of His6-tagged LdhB in Escherichia coli strain BL21(DE3) Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + NADH + H+ Rhizopus arrhizus LdhB enzymes from type-I isolate NRRL 395 and type-II isolate 99-880 show reductive LDH activity, but no oxidative LDH activity, overview (S)-lactate + NAD+
-
ir

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
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strains 99-880 and NRRL 395, genes ldhB and ldhA
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged LdhB from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography and cleavage of the tag Rhizopus arrhizus

Source Tissue

Source Tissue Comment Organism Textmining
additional information type-I isolate, e.g. strain NRRL 395, accumulating primarily lactic acid when grown in the presence of a fermentable carbon source and containing two LDH genes, ldhA and ldhB, and type-II isolates, e.g. strain 99-880, synthesizing predominantly fumaric acid and having only an ldhB gene Rhizopus arrhizus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+ LdhB enzymes from type-I isolate NRRL 395 and type-II isolate 99-880 show reductive LDH activity, but no oxidative LDH activity, overview Rhizopus arrhizus (S)-lactate + NAD+
-
ir
pyruvate + NADH + H+ LdhB Rhizopus arrhizus (S)-lactate + NAD+
-
ir

Synonyms

Synonyms Comment Organism
LdhA
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Rhizopus arrhizus
LDHB
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Rhizopus arrhizus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhizopus arrhizus
NADH
-
Rhizopus arrhizus