Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-phosphoribosyl 1-diphosphate | activates | Thermus caldophilus | |
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Thermus caldophilus | |
fructose 1,6-diphosphate | maximal activation is about: 0.03 mM at pH 6.0, 0.2 mM at pH 7.1, 2 mM at pH 8.0 and 7 mM at pH 9.0 | Thermus caldophilus | |
fructose 2,6-diphosphate | activates | Thermus caldophilus | |
glucose 1,6-diphosphate | activates | Thermus caldophilus | |
phosphate | slight activation of not activated enzyme, inhibition of fructose 1,6-diphosphate activated enzyme | Thermus caldophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | activation at low concentrations, inhibition at high concentrations | Thermus caldophilus | |
phosphate | slight activation of non-activated enzyme, inhibition of fructose 1,6-diphosphate activated enzyme | Thermus caldophilus | |
pyruvate | strong substrate inhibition at high concentrations, fructose 1,6-diphosphate activated enzyme | Thermus caldophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NAD+ | - |
Thermus caldophilus | |
0.065 | - |
NAD+ | - |
Thermus caldophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | Thermus caldophilus | - |
L-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | Thermus caldophilus GK24 | - |
L-lactate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | - |
GK24 | - |
Thermus caldophilus GK24 | - |
GK24 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Thermus caldophilus | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Thermus caldophilus GK24 | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | - |
Thermus caldophilus | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Thermus caldophilus GK24 | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Thermus caldophilus | L-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Thermus caldophilus GK24 | L-lactate + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Thermus caldophilus | |
NADH | coenzyme | Thermus caldophilus |