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Literature summary for 1.1.1.267 extracted from

  • Xu, C.; Wei, H.; Movahedi, A.; Sun, W.; Ma, X.; Li, D.; Yin, T.; Zhuge, Q.
    Evaluation, characterization, expression profiling, and functional analysis of DXS and DXR genes of Populus trichocarpa (2019), Plant Physiol. Biochem., 142, 94-105 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene DXR, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant overexpression in Populus trichocarpa using the transfection method via Agrobacterium tumefaciens strain EHA105 transfecting young leaves and petioles of the Nanlin895 poplar, quantitative reverse transcription PCR enzyme expression analysis, the PtDXR gene is stably integrated into the genomes of the R1-1, R2-4, R3-1, R4-7, R5-3, R6-1, R7-3, and R8-2 lines and leads to expression of the DXR protein using the expression system of the poplar Populus trichocarpa

Protein Variants

Protein Variants Comment Organism
additional information recombinant overexpression of PtDXR in transgenic poplars improves tolerance to abiotic and biotic stresses. Overexpression of PtDXR increases plant tolerance to salt stress, the phenotype, RWC, SOD and POD activities. Increased transcript levels of PtDXR increase in response to Septotinia populiperda, the spread and extent of pathogens in the wild-type plants are faster and greater than in the transgenic lines, based on analysis of the length and width of the largest pathogenic region, phenotype overview Populus trichocarpa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Populus trichocarpa
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2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa A0A2K1XJL6 cv. Nanlin895
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Populus trichocarpa

Source Tissue

Source Tissue Comment Organism Textmining
leaf mature and young Populus trichocarpa
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additional information tissue expression pattern of enzyme DXR, overview Populus trichocarpa
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petiole
-
Populus trichocarpa
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root
-
Populus trichocarpa
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seedling 3-month-old seedlings Populus trichocarpa
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stem upper and lower parts Populus trichocarpa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Populus trichocarpa 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?
additional information product identification by mass spectrometry (HPLC-MS) Populus trichocarpa ?
-
-

Subunits

Subunits Comment Organism
? x * 51800, recombinant His6-tagged enzyme, SDS-PAGE Populus trichocarpa
More a putative conserved cleavage site in the DXR motif is located at the N-terminus of PtDXR, and the NADPH-binding domains are involved in the transformation of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP). Predicted tertiary structures of PtDXR, overview Populus trichocarpa

Synonyms

Synonyms Comment Organism
DXR
-
Populus trichocarpa
PtDXR
-
Populus trichocarpa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Populus trichocarpa
NADPH
-
Populus trichocarpa

Expression

Organism Comment Expression
Populus trichocarpa elicitor treatments such as abscisic acid, NaCl, PEG6000, H2O2, and cold stress show that PtDXR is an elicitor-responsive gene. PtDXR does not exhibit diurnal changes. Overexpression of PtDXR in transgenic poplars improves tolerance to abiotic and biotic stresses. PtDXR expression is increased under the abiotic stress treatments. Treatment with 100 mM abscisic acid (ABA) for 12 h results in a transcript level of PtDXR approximately 20fold greater than the control level. Following treatment with 10% PEG 6000, the highest expression of PtDXR is evident on day 5, while with 4°C cold stress, expression levels are approximately 30fold above the control levels. The expression of PtDXR declines during the first 3 h and then increased, reaching the highest level at 24 h of 2 mM H2O2 treatment. For the abiotic stresses, 200 mM NaCl shows the greatest effect, with an approximately 60fold increase in PtDXR expression at 48 h up

General Information

General Information Comment Organism
malfunction overexpression of PtDXR in transgenic poplars improves tolerance to abiotic and biotic stresses. Increased transcript levels of PtDXR alter the response to Septotinia populiperda. The spread and extent of pathogens in the wild-type plants are faster and greater than in the transgenic lines, based on analysis of the length and width of the largest pathogenic region Populus trichocarpa
metabolism 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) are key enzymes in terpenoid biosynthesis. The first two rate-limiting enzymes in the plastid-located 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway are 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR). The first major step involves DXS-dependent catalysis of pyruvate and D-glyceraldehyde-3-phosphate to form 1-deoxy-D-xylulose-5-phosphate (DXP). The second step is catalyzed by DXR and generates MEP, followed by several enzymatic reactions to produce precursors of IPP and DMAPP. DXS catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate (DXP) from pyruvate and D-glyceraldehyde-3-phosphate. DXR catalyzes the formation of 2-C-methyl-D-erythritol 4-phosphate (MEP) from DXP Populus trichocarpa
physiological function PtDXR encodes a functional protein, and widely participates in plant growth and development, stress physiological process Populus trichocarpa