Literature summary for 1.1.1.267 extracted from

Yan, G.; Zhu, B.R.; Tian, F.L.; Hui, X.; Li, H.; Li, Y.M.; Gao, W.Y.
Inhibitory activity of plant essential oils against E. coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase (2019), Molecules, 24, 2518 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	no effect by 0.5% DMSO on enzyme activity	Escherichia coli

Application

Application	Comment	Organism
additional information	the enzyme is a target for development of antibacterial drugs, determination of the antimicrobial activities of various essential oils against different microbials	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
carvacrol	55.6% inhibition of DXR	Escherichia coli
carveol	23.2% inhibition of DXR	Escherichia coli
eugenol	68.3% inhibition of DXR	Escherichia coli
fosmidomycin	-	Escherichia coli
FR900098	-	Escherichia coli
gallocatechin gallate	strong inhibition of DXR, isolated from Camellia sinenesis	Escherichia coli
linalool	22.7% inhibition of DXR	Escherichia coli
additional information	determination of the antimicrobial activities of various essential oils against different microbials using 35 plant essential oils (EOs), which have long been recognized for their antimicrobial properties. Essential oils of Zanbthoxylum bungeanum (ZB), Schizonepetae tenuifoliae (ST), Thymus quinquecostatus (TQ), Origanum vulgare (OV), and Eugenia caryophyllata (EC) display weak to medium inhibitory activity against DXR, with IC50 values of 0.078 mg/ml, 0.065 mg/ml, 0.059 mg/ml, 0.048 mg/ml, and 0.037 mg/ml, respectively. Dry roots or dry fruits are used for extraction. Cercis siliquastrum leaf extract strongly inhibits enzyme DXR. No effect by 0.5% DMSO on enzyme activity	Escherichia coli
theaflavin-3,3'-digallate	strong inhibition of DXR, isolated from Camellia sinenesis	Escherichia coli
Thymol	26.5% inhibition of DXR	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme requires the presence of a divalent metal ion for activity	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+	Escherichia coli	-	2-C-methyl-D-erythritol 4-phosphate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P45568	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+	-	Escherichia coli	2-C-methyl-D-erythritol 4-phosphate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
DXR	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Escherichia coli
NADPH	-	Escherichia coli

General Information

General Information	Comment	Organism
metabolism	rate-limiting enzyme in the 2-methyl-D-erythritol-4-phosphate (MEP) terpenoid biosynthetic pathway catalyzing the second step	Escherichia coli

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Release 2023.1 (January 2023)