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Literature summary for 1.1.1.267 extracted from

  • Hui, X.; Hua, S.H.; Wu, Q.Q.; Li, H.; Gao, W.Y.
    Antimicrobial mechanism of epigallocatechin gallate and gallocatechin gallate they target 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway (2017), Arch. Biochem. Biophys., 622, 1-8 .
    View publication on PubMed
Search for more literature for 1.1.1.267

Application

Application Comment Organism
drug development enzyme DXR is a validated antimicrobial target Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
baicalein
-
 Escherichia coli
epigallocatechin gallate specifically inhibits the enzyme and has antimicrobial activity, competitive inhibition versus DXP and uncompetitive inhibition versus NADPH Escherichia coli
gallocatechin gallate specifically inhibits the enzyme and has antimicrobial activity, competitive inhibition versus DXP and uncompetitive inhibition versus NADPH Escherichia coli
additional information possible interactions between DXR and the catechine inhibitors are simulated via molecular docking simulation, detailed overview. Triton X-100 does not affect the inhibition of the enzyme by epigallocatechin gallate and gallocatechin gallate, but that by baicalein (positive control) Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P45568
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
1-deoxy-D-xylulose 5-phosphate reductoisomerase
-
 Escherichia coli
DXR
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADPH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0234
-
 gallocatechin gallate pH 7.0, 37°C, recombinant enzyme, uncompetitive versus NADPH Escherichia coli
0.0252
-
 gallocatechin gallate pH 7.0, 37°C, recombinant enzyme, competitive versus 1-deoxy-D-xylulose 5-phosphate Escherichia coli
0.1483
-
 epigallocatechin gallate pH 7.0, 37°C, recombinant enzyme, competitive versus 1-deoxy-D-xylulose 5-phosphate Escherichia coli
0.1794
-
 epigallocatechin gallate pH 7.0, 37°C, recombinant enzyme, uncompetitive versus NADPH Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0275
-
 pH 7.0, 37°C, recombinant enzyme Escherichia coli gallocatechin gallate
0.2098
-
 pH 7.0, 37°C, recombinant enzyme Escherichia coli epigallocatechin gallate

General Information

General Information Comment Organism
metabolism 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) is the first key enzyme in the MEP pathway for terpenoid biosynthesis Escherichia coli