Cloned (Comment) | Organism |
---|---|
gene DXR, sequence comparisons and phylogenetic analysis and tree, semi-quantitative RT-PCR and quantitative real-time PCR expression analysis, recombinant expression | Centella asiatica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme sequence contains a conserved transit peptide for plastid motif Cys-Ser-(Ala/Met/Val/Thr) | Centella asiatica | 9507 | - |
additional information | Centella DXR transcript is localized in chloroplasts and other plastids | Centella asiatica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Centella asiatica | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Centella asiatica | A0A0A6Z621 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Centella asiatica | - |
additional information | CaDXR is differentially expressed in different tissues with maximal expression in the node and lowest in the roots, Centella DXR transcript is expressed in vegetative tissues | Centella asiatica | - |
node | high expression level | Centella asiatica | - |
root | low expression level | Centella asiatica | - |
stem | - |
Centella asiatica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Centella asiatica | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51500, SDS-PAGE | Centella asiatica |
More | enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. CaDXR is a basically alpha-helical protein which contains 21 alpha-helixes, 11 beta-sheets, and one hairpin structure | Centella asiatica |
Synonyms | Comment | Organism |
---|---|---|
CaDXR | - |
Centella asiatica |
DXR | - |
Centella asiatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor docking study with DXR, presence of conserved NADPH-binding motif (GSTGSIGT and LAAGSNV) in N-terminal end of the CaDXR protein sequence | Centella asiatica | |
NADP+ | - |
Centella asiatica | |
NADPH | - |
Centella asiatica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Centella asiatica | isoelectric focusing | - |
6.33 |
General Information | Comment | Organism |
---|---|---|
metabolism | the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is the rate-limiting enzyme in the methylerythritol 4-phosphate pathway (MEP) via conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP | Centella asiatica |
additional information | enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. Three-dimensional molecular modeling and model validation, and molecular dynamics simulation of modeled DXR, overview | Centella asiatica |
physiological function | the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), an NADPH-dependent reductase, plays a pivotal role in the methylerythritol 4-phosphate pathway (MEP), in the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP. Structure-function analysis of DXR and regulatory mechanism, molecular dynamics simulations, overview | Centella asiatica |