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Literature summary for 1.1.1.267 extracted from

  • Cai, G.; Deng, L.; Xue, J.; Moreno, S.N.; Striepen, B.; Song, Y.
    Expression, characterization and inhibition of Toxoplasma gondii 1-deoxy-D-xylulose-5-phosphate reductoisomerase (2013), Bioorg. Med. Chem. Lett., 23, 2158-2161.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged enzyme expression in Escherichia coli BL21-CodonPlus strain Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
(pyridin-2-ylmethyl)phosphonic acid
-
Toxoplasma gondii
1-hydroxy-5-phenylpyridin-2(1H)-one
-
Toxoplasma gondii
fosmidomycin potent inhibitor but with very short half-life in plasma and low oral availability Toxoplasma gondii
FR900098
-
Toxoplasma gondii
additional information inhibitor design and synthesis, structure-activity relationship profile for the inhibition of TgDXR, overview Toxoplasma gondii
[(5-phenylpyridin-2-yl)methyl]phosphonic acid
-
Toxoplasma gondii
[1-(3,4-dichlorophenyl)-3-[formyl(hydroxy)amino]propyl]phosphonic acid
-
Toxoplasma gondii
[3-[acetyl(hydroxy)amino]-1-(3,4-dichlorophenyl)propyl]phosphonic acid
-
Toxoplasma gondii
[3-[acetyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid
-
Toxoplasma gondii
[3-[acetyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid
-
Toxoplasma gondii
[3-[formyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid
-
Toxoplasma gondii
[3-[formyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid
-
Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0255
-
1-deoxy-D-xylulose 5-phosphate pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially substitute for Mg2+ or Mn2+ Toxoplasma gondii
Mg2+ the enzyme essentially requires Mg2+ or Mn2+ Toxoplasma gondii
Mn2+ the enzyme essentially requires Mg2+ or Mn2+ Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, about, recombinant His6-tagged enzyme, SDS-PAGE Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Toxoplasma gondii
-
2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli BL21-CodonPlus strain by nickel affinity chromatography Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Toxoplasma gondii 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 45000, about, recombinant His6-tagged enzyme, SDS-PAGE Toxoplasma gondii

Synonyms

Synonyms Comment Organism
1-deoxy-D-xylulose-5-phosphate
-
Toxoplasma gondii
DXR
-
Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Toxoplasma gondii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000048
-
FR900098 pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.000055
-
[3-[formyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.000077
-
[1-(3,4-dichlorophenyl)-3-[formyl(hydroxy)amino]propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.000079
-
[3-[formyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.00009
-
fosmidomycin pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.00022
-
[3-[acetyl(hydroxy)amino]-1-(3,4-dichlorophenyl)propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.00053
-
[3-[acetyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.00097
-
[3-[acetyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.0021
-
[(5-phenylpyridin-2-yl)methyl]phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.0041
-
(pyridin-2-ylmethyl)phosphonic acid pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii
0.0256
-
1-hydroxy-5-phenylpyridin-2(1H)-one pH 7.6, 30°C, recombinant enzyme Toxoplasma gondii

General Information

General Information Comment Organism
physiological function 1-deoxy-D-xylulose-5-phosphate reductoisomerase in the non-mevalonate isoprene biosynthesis pathway is essential to the organism Toxoplasma gondii