Cloned (Comment) | Organism |
---|---|
His6-tagged enzyme expression in Escherichia coli BL21-CodonPlus strain | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(pyridin-2-ylmethyl)phosphonic acid | - |
Toxoplasma gondii | |
1-hydroxy-5-phenylpyridin-2(1H)-one | - |
Toxoplasma gondii | |
fosmidomycin | potent inhibitor but with very short half-life in plasma and low oral availability | Toxoplasma gondii | |
FR900098 | - |
Toxoplasma gondii | |
additional information | inhibitor design and synthesis, structure-activity relationship profile for the inhibition of TgDXR, overview | Toxoplasma gondii | |
[(5-phenylpyridin-2-yl)methyl]phosphonic acid | - |
Toxoplasma gondii | |
[1-(3,4-dichlorophenyl)-3-[formyl(hydroxy)amino]propyl]phosphonic acid | - |
Toxoplasma gondii | |
[3-[acetyl(hydroxy)amino]-1-(3,4-dichlorophenyl)propyl]phosphonic acid | - |
Toxoplasma gondii | |
[3-[acetyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid | - |
Toxoplasma gondii | |
[3-[acetyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid | - |
Toxoplasma gondii | |
[3-[formyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid | - |
Toxoplasma gondii | |
[3-[formyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid | - |
Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0255 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially substitute for Mg2+ or Mn2+ | Toxoplasma gondii | |
Mg2+ | the enzyme essentially requires Mg2+ or Mn2+ | Toxoplasma gondii | |
Mn2+ | the enzyme essentially requires Mg2+ or Mn2+ | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, about, recombinant His6-tagged enzyme, SDS-PAGE | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Toxoplasma gondii | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli BL21-CodonPlus strain by nickel affinity chromatography | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Toxoplasma gondii | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, about, recombinant His6-tagged enzyme, SDS-PAGE | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose-5-phosphate | - |
Toxoplasma gondii |
DXR | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Toxoplasma gondii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000048 | - |
FR900098 | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.000055 | - |
[3-[formyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.000077 | - |
[1-(3,4-dichlorophenyl)-3-[formyl(hydroxy)amino]propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.000079 | - |
[3-[formyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.00009 | - |
fosmidomycin | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.00022 | - |
[3-[acetyl(hydroxy)amino]-1-(3,4-dichlorophenyl)propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.00053 | - |
[3-[acetyl(hydroxy)amino]-1-(pyridin-4-yl)propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.00097 | - |
[3-[acetyl(hydroxy)amino]-1-(pyridin-3-yl)propyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.0021 | - |
[(5-phenylpyridin-2-yl)methyl]phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.0041 | - |
(pyridin-2-ylmethyl)phosphonic acid | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii | |
0.0256 | - |
1-hydroxy-5-phenylpyridin-2(1H)-one | pH 7.6, 30°C, recombinant enzyme | Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
physiological function | 1-deoxy-D-xylulose-5-phosphate reductoisomerase in the non-mevalonate isoprene biosynthesis pathway is essential to the organism | Toxoplasma gondii |