Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression pattern analysis, functional recombinant expression under the control of CaMV 35S promoter in Nicotiana tabacum cv. Zhongyan 90 via Agrobacterium-mediated transformation Overexpression of AvDXR in tobacco increases DXRactivity, photosynthetic pigment content and volatile isoprenoid components | Wurfbainia villosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | a putative chloroplast transit peptide with a conserved cleavage site of CS-M motif is located at the N-terminus of AvDXR | Wurfbainia villosa | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Wurfbainia villosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Wurfbainia villosa | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wurfbainia villosa | C6G1Y8 | from medicinal garden of Guangzhou University of Chinese Medicine, China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | higher enzyme level | Wurfbainia villosa | - |
leaf | - |
Wurfbainia villosa | - |
additional information | semi-quantitative RT-PCR expression pattern analysis | Wurfbainia villosa | - |
pericarp | - |
Wurfbainia villosa | - |
root | - |
Wurfbainia villosa | - |
seedling | - |
Wurfbainia villosa | - |
stem | - |
Wurfbainia villosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Wurfbainia villosa | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose-5-phosphate reductoisomerase | - |
Wurfbainia villosa |
DXR | - |
Wurfbainia villosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Wurfbainia villosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the NADPH-binding domains are critical to the catalytic activity of DXR. Two highly conserved NADPH-binding domains, GSTGSIGT and LAAGSNV, locate in the N-terminal region of AvDXR | Wurfbainia villosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second regulation step in the 2-C-methyl-D-erythritol 4-phosphate pathway and the the first committed step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis | Wurfbainia villosa |
additional information | two substrate-binding domains, LPADSEHSAI and NKGLEVIEAHY, locate in its mid-region of the enzyme molecule, which is highly homologous to DXRs from other plants and similar to the DXR from Escherichia coli | Wurfbainia villosa |