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Literature summary for 1.1.1.267 extracted from

  • Yang, J.; Adhikari, M.; Liu, H.; Xu, H.; He, G.; Zhan, R.; Wei, J.; Chen, W.
    Characterization and functional analysis of the genes encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase, the two enzymes in the MEP pathway, from Amomum villosum Lour (2012), Mol. Biol. Rep., 39, 8287-8296.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression pattern analysis, functional recombinant expression under the control of CaMV 35S promoter in Nicotiana tabacum cv. Zhongyan 90 via Agrobacterium-mediated transformation Overexpression of AvDXR in tobacco increases DXRactivity, photosynthetic pigment content and volatile isoprenoid components Wurfbainia villosa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast a putative chloroplast transit peptide with a conserved cleavage site of CS-M motif is located at the N-terminus of AvDXR Wurfbainia villosa 9507
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Wurfbainia villosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Wurfbainia villosa
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2-C-methyl-D-erythritol 4-phosphate + NADP+
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?

Organism

Organism UniProt Comment Textmining
Wurfbainia villosa C6G1Y8 from medicinal garden of Guangzhou University of Chinese Medicine, China
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Source Tissue

Source Tissue Comment Organism Textmining
fruit higher enzyme level Wurfbainia villosa
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leaf
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Wurfbainia villosa
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additional information semi-quantitative RT-PCR expression pattern analysis Wurfbainia villosa
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pericarp
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Wurfbainia villosa
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root
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Wurfbainia villosa
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seedling
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Wurfbainia villosa
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stem
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Wurfbainia villosa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
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Wurfbainia villosa 2-C-methyl-D-erythritol 4-phosphate + NADP+
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?

Synonyms

Synonyms Comment Organism
1-deoxy-D-xylulose-5-phosphate reductoisomerase
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Wurfbainia villosa
DXR
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Wurfbainia villosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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assay at Wurfbainia villosa

Cofactor

Cofactor Comment Organism Structure
NADPH the NADPH-binding domains are critical to the catalytic activity of DXR. Two highly conserved NADPH-binding domains, GSTGSIGT and LAAGSNV, locate in the N-terminal region of AvDXR Wurfbainia villosa

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the second regulation step in the 2-C-methyl-D-erythritol 4-phosphate pathway and the the first committed step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis Wurfbainia villosa
additional information two substrate-binding domains, LPADSEHSAI and NKGLEVIEAHY, locate in its mid-region of the enzyme molecule, which is highly homologous to DXRs from other plants and similar to the DXR from Escherichia coli Wurfbainia villosa