Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.267 extracted from

  • Dhiman, R.K.; Schaeffer, M.L.; Bailey, A.M.; Testa, C.A.; Scherman, H.; Crick, D.C.
    1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin (2005), J. Bacteriol., 187, 8395-8402.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ispC, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), Rv2870c complements an inactivated chromosomal copy of IspC in Salmonella enterica, and the complemented strain is sensitive to fosmidomycin Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
1-deoxy-D-xylulose 5-phosphate substrate inhibition Mycobacterium tuberculosis
EDTA complete inhibition at 5 mM Mycobacterium tuberculosis
fosmidomycin IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteria and other organisms Mycobacterium tuberculosis
additional information no substrate inhibition by 2-C-methyl-D-erythritol 4-phosphate, NADPH or NADP+ Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0297
-
NADPH pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
0.0471
-
1-deoxy-D-xylulose 5-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
0.174
-
2-C-methyl-D-erythritol 4-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
0.561
-
NADP+ pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mycobacterium tuberculosis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely dependent on, optimal at 4 mM Mycobacterium tuberculosis
additional information Mn2+, Ca2+, Co2+, Zn2+, and Fe2+ ions are unable to support activity Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis H37Rv first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
gene ispC
-
Mycobacterium tuberculosis H37Rv
-
gene ispC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 45000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
IspC
-
Mycobacterium tuberculosis
Rv2870c
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.9 forward and reverse reaction Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Mycobacterium tuberculosis
NADPH
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1
-
1-deoxy-D-xylulose 5-phosphate pH 7.9, 30°C, recombinant His-tagged enzyme Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00031
-
IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri Mycobacterium tuberculosis fosmidomycin
0.0027
-
IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri Mycobacterium tuberculosis fosmidomycin