Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E231K | less than 0.24% wild type kcat | Escherichia coli |
G14D | mutant | Escherichia coli |
H153Q | increase of Km | Escherichia coli |
H209Q | increase of Km | Escherichia coli |
H257Q | increase of Km | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADPH | with 1 mM MnCl2 | Escherichia coli | |
0.009 | - |
NADPH | with 1 mM CoCl2 | Escherichia coli | |
0.02 | - |
NADPH | with 1 mM MgCl2 | Escherichia coli | |
0.06 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM CoCl2 | Escherichia coli | |
0.099 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM MgCl2 | Escherichia coli | |
0.25 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM MnCl2 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for activity | Escherichia coli | |
Mg2+ | required for activity | Escherichia coli | |
Mn2+ | required for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Escherichia coli | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P45568 | - |
- |
Purification (Comment) | Organism |
---|---|
of recombinant protein | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 100 mM Tris-HCl pH 7.5, 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
not heat-stable above | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |