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Literature summary for 1.1.1.267 extracted from

  • Kuzuyama, T.; Takahashi, S.; Takagi, M.; Seto, H.
    Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues (2000), J. Biol. Chem., 275, 19928-19932.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
E231K less than 0.24% wild type kcat Escherichia coli
G14D mutant Escherichia coli
H153Q increase of Km Escherichia coli
H209Q increase of Km Escherichia coli
H257Q increase of Km Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
fosmidomycin
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADPH with 1 mM MnCl2 Escherichia coli
0.009
-
NADPH with 1 mM CoCl2 Escherichia coli
0.02
-
NADPH with 1 mM MgCl2 Escherichia coli
0.06
-
1-deoxy-D-xylulose 5-phosphate with 1 mM CoCl2 Escherichia coli
0.099
-
1-deoxy-D-xylulose 5-phosphate with 1 mM MgCl2 Escherichia coli
0.25
-
1-deoxy-D-xylulose 5-phosphate with 1 mM MnCl2 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required for activity Escherichia coli
Mg2+ required for activity Escherichia coli
Mn2+ required for activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli
-
2-C-methyl-D-erythritol 4-phosphate + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli P45568
-
-

Purification (Commentary)

Purification (Comment) Organism
of recombinant protein Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 100 mM Tris-HCl pH 7.5, 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
not heat-stable above Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli