Cloned (Comment) | Organism |
---|---|
gene YMR152W, sequence comparison and phylogenetic analysis | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibition level depends on the substrate used, about 40% inhibition at 10 mM | Saccharomyces cerevisiae | |
Ag+ | - |
Saccharomyces cerevisiae | |
Al3+ | - |
Saccharomyces cerevisiae | |
Ca2+ | slight inhibition at 2 mM, slight activation at 0.5 mM, dependent on the substrate | Saccharomyces cerevisiae | |
Co2+ | - |
Saccharomyces cerevisiae | |
Cu2+ | - |
Saccharomyces cerevisiae | |
Fe3+ | - |
Saccharomyces cerevisiae | |
KCl | - |
Saccharomyces cerevisiae | |
Mg2+ | slight inhibition at 2 mM, slight activation at 0.5 mM, dependent on the substrate | Saccharomyces cerevisiae | |
Mn2+ | - |
Saccharomyces cerevisiae | |
additional information | the strength of inhibition by metal ions depends on the substrate used, overview | Saccharomyces cerevisiae | |
NaCl | - |
Saccharomyces cerevisiae | |
Ni2+ | - |
Saccharomyces cerevisiae | |
Zn2+ | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
acetaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae | |
16.11 | - |
glycolaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae | |
97.37 | - |
furfural | pH 7.0, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
lipid droplet | - |
Saccharomyces cerevisiae | 5811 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition at 2 mM, slight activation at 0.5 mM, dependent on the substrate | Saccharomyces cerevisiae | |
Mg2+ | slight inhibition at 2 mM, slight activation at 0.5 mM, dependent on the substrate | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylbutanal + NADPH + H+ | Saccharomyces cerevisiae | - |
3-methylbutanol + NADP+ | - |
r | |
5-hydroxymethylfurfural + NADH + H+ | Saccharomyces cerevisiae | - |
2,5-dihydroxymethylfurane + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | Saccharomyces cerevisiae | - |
ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | Saccharomyces cerevisiae ATCC 204508 | - |
ethanol + NAD+ | - |
r | |
benzaldehyde + NADPH + H+ | Saccharomyces cerevisiae | - |
benzylalcohol + NADP+ | - |
r | |
benzaldehyde + NADPH + H+ | Saccharomyces cerevisiae ATCC 204508 | - |
benzylalcohol + NADP+ | - |
r | |
furfural + NADH + H+ | Saccharomyces cerevisiae | - |
furfuryl alcohol + NAD+ | - |
r | |
furfural + NADH + H+ | Saccharomyces cerevisiae ATCC 204508 | - |
furfuryl alcohol + NAD+ | - |
r | |
glycolaldehyde + NADH + H+ | Saccharomyces cerevisiae | - |
ethylene glycol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P28625 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | P28625 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.74 | - |
substrate 3-methylbutanal, pH 7.0, 30°C | Saccharomyces cerevisiae |
1.12 | - |
substrate benzaldehyde, pH 7.0, 30°C | Saccharomyces cerevisiae |
1.74 | - |
substrate 5-hydroxymethylfurfural, pH 7.0, 30°C | Saccharomyces cerevisiae |
5.05 | - |
substrate furfural, pH 7.0, 30°C | Saccharomyces cerevisiae |
9.64 | - |
substrate glycolaldehyde, pH 7.0, 30°C | Saccharomyces cerevisiae |
190.86 | - |
substrate acetaldegyde, pH 7.0, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylbutanal + NADPH + H+ | - |
Saccharomyces cerevisiae | 3-methylbutanol + NADP+ | - |
r | |
3-methylbutanal + NADPH + H+ | low activity | Saccharomyces cerevisiae | 3-methylbutanol + NADP+ | - |
r | |
5-hydroxymethylfurfural + NADH + H+ | - |
Saccharomyces cerevisiae | 2,5-dihydroxymethylfurane + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | best substrate | Saccharomyces cerevisiae | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | best substrate | Saccharomyces cerevisiae ATCC 204508 | ethanol + NAD+ | - |
r | |
benzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | benzylalcohol + NADP+ | - |
r | |
benzaldehyde + NADPH + H+ | low activity | Saccharomyces cerevisiae | benzylalcohol + NADP+ | - |
r | |
benzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | benzylalcohol + NADP+ | - |
r | |
furfural + NADH + H+ | - |
Saccharomyces cerevisiae | furfuryl alcohol + NAD+ | - |
r | |
furfural + NADH + H+ | - |
Saccharomyces cerevisiae ATCC 204508 | furfuryl alcohol + NAD+ | - |
r | |
glycolaldehyde + NADH + H+ | - |
Saccharomyces cerevisiae | ethylene glycol + NAD+ | - |
r | |
additional information | Ymr152wp catalyzes reactions for reduction of acetaldehyde, glycolaldehyde, furfural, and 5-hydroxymethylfurfural (HMF) when NADH is used as the cofactor. Besides, enzyme activity is detected for reduction of benzaldehyde (BZA) and 3-methylbutanal (MBA) when NADPH is used as the cofactor. Ymr152wp shows the highest specific enzyme activity (190.86 U/mg) for reduction of acetaldehyde, followed by glycolaldehyde (9.64 U/mg), furfural (5.05 U/mg), HMF (1.74 U/mg), BZA (1.12 U/mg), and MBA (0.74 U/mg). No activity with formaldehyde, propionaldehyde, butyraldehyde, glutaraldehyde, quinone, 1,2-naphthoquinone, 9,10-phenanthrenequinone, 4-benzoquinone, acetone, and acetylacetone, neither with NADH, nor with NADPH | Saccharomyces cerevisiae | ? | - |
- |
|
additional information | Ymr152wp catalyzes reactions for reduction of acetaldehyde, glycolaldehyde, furfural, and 5-hydroxymethylfurfural (HMF) when NADH is used as the cofactor. Besides, enzyme activity is detected for reduction of benzaldehyde (BZA) and 3-methylbutanal (MBA) when NADPH is used as the cofactor. Ymr152wp shows the highest specific enzyme activity (190.86 U/mg) for reduction of acetaldehyde, followed by glycolaldehyde (9.64 U/mg), furfural (5.05 U/mg), HMF (1.74 U/mg), BZA (1.12 U/mg), and MBA (0.74 U/mg). No activity with formaldehyde, propionaldehyde, butyraldehyde, glutaraldehyde, quinone, 1,2-naphthoquinone, 9,10-phenanthrenequinone, 4-benzoquinone, acetone, and acetylacetone, neither with NADH, nor with NADPH | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
aldehyde reductase | - |
Saccharomyces cerevisiae |
protein YIM1 | - |
Saccharomyces cerevisiae |
YIM1 | - |
Saccharomyces cerevisiae |
YMR152W | - |
Saccharomyces cerevisiae |
Ymr152wp | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 70% of maximal activity at 20°C, maximal activity at 25-30°C, low activity at 60°C | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
Ymr152wp completely loses its catalytic activities for reduction of furfural after incubation at 60°C for 15 min, and for reduction of acetaldehyde and glycolaldehyde after incubation at 60°C for 30 min | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.44 | - |
glycolaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae | |
36.9 | - |
furfural | pH 7.0, 30°C | Saccharomyces cerevisiae | |
313.14 | - |
acetaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | optimum pH of Ymr152wp is acidic at pH 5.0-6.0, but this enzyme is more stable in alkaline conditions at pH 8.0, relative activity of Ymr152wp drops quickly under alkaline conditions from pH 7.0 to pH 9.0. The highest enzyme activity of Ymr152wp is observed at pH 5.5 with furfural as substrate, 15% of maximal activity at pH 9.0, 40% at pH 6.5. The optimum pH for reduction of glycolaldehyde is pH 6.0.showing more than 80% of its maximal enzyme activity at pH 4.5-8.5 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
optimum pH of Ymr152wp is acidic at pH 5.0-6.0, but this enzyme is more stable in alkaline conditions at pH 8.0 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae | |
NADP+ | - |
Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis indicates that Ymr152wp and selected proteins similar to Ymr152wp are classified into the MDR family, which is close to the QOR subfamily and the LTD subfamily, but is far from the zinc-containing subfamilies of the ADH subfamily, the CADH subfamily, and the YADH subfamily in the genetic tree | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
furfural | pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.96 | - |
glycolaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae | |
423.16 | - |
acetaldehyde | pH 7.0, 30°C | Saccharomyces cerevisiae |