Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 5-6fold increasing activity for NADPH-dependent activity at 0.2 M | Saccharomyces cerevisiae | |
NaCl | 5-6fold increasing activity for NADPH-dependent activity at 0.2 M | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
nutrition | essential in removal of the worthy off-flavours in beer during fermentation | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methylbutanal | presence of NADPH, Ki: 2.74 mM, presence of NADH, Ki: 4.61 mM | Saccharomyces cerevisiae | |
3-Methylbutanal | presence of NADPH, Ki: 7.14 mM, presence of NADH, Ki: 253 mM | Saccharomyces cerevisiae | |
Butanal | presence of NADPH, Ki: 16.6 mM | Saccharomyces cerevisiae | |
Heptanal | presence of NADPH, Ki: 0.88 mM, presence of NADH, Ki: 1.17 mM | Saccharomyces cerevisiae | |
hexanal | presence of NADPH, Ki: 0.79 mM, presence of NADH, Ki: 2.8 mM | Saccharomyces cerevisiae | |
KCl | 5-6fold decreasing activity at 0.2 M for NADH-dependent activity | Saccharomyces cerevisiae | |
NaCl | 5-6fold decreasing activity at 0.2 M for NADH-dependent activity | Saccharomyces cerevisiae | |
NADP+ | complete inhibition at a 6.7fold excess of NADP+ in a reduction assay with 0.15 mM NADH | Saccharomyces cerevisiae | |
pentanal | presence of NADPH, Ki: 3.9 mM, presence of NADH, Ki: 13.9 mM | Saccharomyces cerevisiae | |
propanal | presence of NADPH, Ki: 46.6 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | - |
Saccharomyces cerevisiae | |
0.16 | - |
pentanal | presence of NADPH | Saccharomyces cerevisiae | |
0.18 | - |
hexanal | presence of NADPH | Saccharomyces cerevisiae | |
0.21 | - |
3-Methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
0.27 | - |
Heptanal | presence of NADPH | Saccharomyces cerevisiae | |
0.83 | - |
hexanal | presence of NADH | Saccharomyces cerevisiae | |
1.22 | - |
Hexanol | presence of NADP+ | Saccharomyces cerevisiae | |
1.69 | - |
trans-2-hexenol | presence of NADP+ | Saccharomyces cerevisiae | |
1.7 | - |
NADH | - |
Saccharomyces cerevisiae | |
1.85 | - |
2-methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
1.89 | - |
3-Methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
2.76 | - |
Butanal | presence of NADPH | Saccharomyces cerevisiae | |
3.01 | - |
pentanal | presence of NADH | Saccharomyces cerevisiae | |
4.25 | - |
Heptanal | presence of NADH | Saccharomyces cerevisiae | |
17.7 | - |
2-methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
23.1 | - |
Butanal | presence of NADH | Saccharomyces cerevisiae | |
27 | - |
propanal | presence of NADH | Saccharomyces cerevisiae | |
38.9 | - |
propanal | presence of NADPH | Saccharomyces cerevisiae | |
158 | - |
acetaldehyde | presence of NADPH | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylbutanal + NAD(P)H | Saccharomyces cerevisiae | - |
3-methylbutanol + NAD(P)+ | - |
r | |
acetaldehyde + NADPH + H+ | Saccharomyces cerevisiae | - |
ethanol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
brewer's yeast | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.6 | - |
in the presence of NADPH | Saccharomyces cerevisiae |
93.9 | - |
in the presence of NADH | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-pentanedione + NAD(P)H | very low activity | Saccharomyces cerevisiae | ? + NAD(P)+ | - |
? | |
2-methylbutanal + NAD(P)H | - |
Saccharomyces cerevisiae | 2-methylbutanol + NAD(P)+ | - |
r | |
3-methylbutanal + NAD(P)H | - |
Saccharomyces cerevisiae | 3-methylbutanol + NAD(P)+ | - |
r | |
3-methylbutanal + NADH + H+ | - |
Saccharomyces cerevisiae | 3-methylbutanol + NAD+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ethanol + NADP+ | - |
r | |
benzaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | benzyl alcohol + NAD(P)+ | - |
? | |
butanal + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | butanol + NAD(P)+ | - |
r | |
diacetyl + NAD(P)H | very low activity | Saccharomyces cerevisiae | ? + NAD(P)+ | - |
? | |
furaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | furfuryl alcohol + NAD(P)+ | - |
? | |
heptanal + NADPH | preference for long and branched-chain substrates with up to seven carbon atoms | Saccharomyces cerevisiae | heptanol + NADP+ | - |
r | |
hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae | hexanol + NADP+ | - |
r | |
p-anisaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | p-anisalcohol + NAD(P)+ | - |
? | |
pentanal + NADPH | - |
Saccharomyces cerevisiae | pentanol + NADP+ | - |
r | |
propanal + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | propanol + NAD(P)+ | - |
r | |
trans-2-hexenol + NADP+ | - |
Saccharomyces cerevisiae | trans-2-hexenal + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
bcADH | - |
Saccharomyces cerevisiae |
branched-chain alcohol dehydrogenase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.55 | - |
Hexanol | in the presence of NADP+ | Saccharomyces cerevisiae | |
5.6 | - |
acetaldehyde | in the presence of NADPH | Saccharomyces cerevisiae | |
14 | - |
trans-2-hexenol | in the presence of NADP+ | Saccharomyces cerevisiae | |
47.3 | - |
Butanal | presence of NADH | Saccharomyces cerevisiae | |
50.1 | - |
hexanal | presence of NADH | Saccharomyces cerevisiae | |
55 | - |
propanal | presence of NADPH | Saccharomyces cerevisiae | |
56.6 | - |
pentanal | presence of NADPH | Saccharomyces cerevisiae | |
57 | - |
Butanal | presence of NADPH | Saccharomyces cerevisiae | |
71.1 | - |
hexanal | presence of NADPH | Saccharomyces cerevisiae | |
72.9 | - |
Heptanal | presence of NADPH | Saccharomyces cerevisiae | |
74.9 | - |
3-Methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
81.6 | - |
pentanal | presence of NADH | Saccharomyces cerevisiae | |
84 | - |
propanal | presence of NADH | Saccharomyces cerevisiae | |
91.8 | - |
3-Methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
92.3 | - |
2-methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
101 | - |
Heptanal | presence of NADH | Saccharomyces cerevisiae | |
113 | - |
2-methylbutanal | presence of NADPH | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | NADH-dependent activity, highest activity at low ionic strength | Saccharomyces cerevisiae |
8.5 | - |
NADPH-dependent activity, highest activity at high ionic strength | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharomyces cerevisiae | |
NADPH | preferred cofactor | Saccharomyces cerevisiae |