Literature summary for 1.1.1.262 extracted from
Cane, D.E.; Du, S.; Robinson, J.K.; Hsiung, Y.; Spenser, I.D.
Biosynthesis of vitamin B6: enzymatic conversion of 1-deoxy-D-xylulose-5-phosphate to pyridoxol phosphate (1999), J. Am. Chem. Soc., 121, 7722-7723.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
overexpression in Escherichia coli |
Escherichia coli |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
EDTA |
inhibition at 1 mM, fully restored by addition of 1 mM Mn2+, Co2+, Mg2+ or Ca2+, partially restored by 1 mM Ni2+ or Zn2+ |
Escherichia coli |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
4-(phosphohydroxy)-L-threonine + NAD+ |
Escherichia coli |
involved in pyridoxal 5'-phosphate formation |
3-phosphohydroxy-1aminoacetone + CO2 + NADH + H+ |
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate |
r |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
to homogeneity, recombinant enzyme |
Escherichia coli |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
additional information |
pdxA gene product |
Escherichia coli |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
4-(phosphohydroxy)-L-threonine + NAD+ |
involved in pyridoxal 5'-phosphate formation |
Escherichia coli |
3-phosphohydroxy-1aminoacetone + CO2 + NADH + H+ |
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate |
r |
|