Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum calidifontis |
Crystallization (Comment) | Organism |
---|---|
to 2.45 A resolution, one homohexamer per asymmetric unit, with presence of the bound cofactor NADPH in subunits D, E, and F. The phosphate group at C2' of the adenine ribose of NADPH is tightly held through the five biased hydrogen bonds with Ser40 and Thr42. The NADPH molecule is pushed away from the normal coenzyme binding site | Pyrobaculum calidifontis |
Protein Variants | Comment | Organism |
---|---|---|
S40A/T42A | increase in activity with NADPH | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.031 | - |
NADPH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.077 | - |
NADH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.1 | - |
NADH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.31 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.72 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.75 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
1.1 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MTM6 | - |
- |
Pyrobaculum calidifontis VA1 | A3MTM6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NADH + H+ | - |
Pyrobaculum calidifontis | sn-glycerol-1-phosphate + NAD+ | - |
? | |
dihydroxyacetone phosphate + NADH + H+ | - |
Pyrobaculum calidifontis VA1 | sn-glycerol-1-phosphate + NAD+ | - |
? | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Pyrobaculum calidifontis | sn-glycerol-1-phosphate + NADP+ | - |
? | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Pyrobaculum calidifontis VA1 | sn-glycerol-1-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EgsA | - |
Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
NADPH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis | |
0.3 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
5.22 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
7 | - |
NADPH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis | |
10.4 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
10.4 | - |
NADH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis | |
10.4 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
11 | - |
NADH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | strong preference for NADH | Pyrobaculum calidifontis | |
NADPH | 2.4% of the reaction rate with NADH | Pyrobaculum calidifontis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
7.29 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADPH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
8.71 | - |
NADPH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis | |
9.39 | - |
dihydroxyacetone phosphate | wild-type, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
14 | - |
dihydroxyacetone phosphate | mutant S40A/T42A, cosubstrate NADH, pH 7.0, 50°C | Pyrobaculum calidifontis | |
101 | - |
NADH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis | |
142 | - |
NADH | wild-type, pH 7.0, 50°C | Pyrobaculum calidifontis | |
1155 | - |
NADPH | mutant S40A/T42A, pH 7.0, 50°C | Pyrobaculum calidifontis |