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Literature summary for 1.1.1.261 extracted from

  • Koga, Y.; Kyuragi, T.; Nishihara, M.; Sone, N.
    Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines descent (1998), J. Mol. Evol., 46, 54-63.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.17
-
dihydroxyacetone phosphate in the presence of NADH Methanothermobacter thermautotrophicus
16.3
-
glycerol-1-phosphate in the presence of NADH Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
302000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H Methanothermobacter thermautotrophicus formation of glycerol-1-phosphate is the natural direction of the reaction sn-glycerol-1-phosphate + NAD(P)+
-
r
dihydroxyacetone phosphate + NAD(P)H Methanothermobacter thermautotrophicus DELTAH formation of glycerol-1-phosphate is the natural direction of the reaction sn-glycerol-1-phosphate + NAD(P)+
-
r
sn-glycerol-1-phosphate + NAD+ Methanothermobacter thermautotrophicus
-
dihydroxyacetone phosphate + NADH
-
r
sn-glycerol-1-phosphate + NAD+ Methanothermobacter thermautotrophicus DELTAH
-
dihydroxyacetone phosphate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus DELTAH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 7step chromatography Methanothermobacter thermautotrophicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Methanothermobacter thermautotrophicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H formation of glycerol-1-phosphate is the natural direction of the reaction Methanothermobacter thermautotrophicus sn-glycerol-1-phosphate + NAD(P)+
-
r
dihydroxyacetone phosphate + NAD(P)H formation of glycerol-1-phosphate is the natural direction of the reaction Methanothermobacter thermautotrophicus DELTAH sn-glycerol-1-phosphate + NAD(P)+
-
r
dihydroxyacetonephosphate + NAD(P)H
-
Methanothermobacter thermautotrophicus glycerol-1-phosphate + NAD(P)+
-
?
dihydroxyacetonephosphate + NAD(P)H
-
Methanothermobacter thermautotrophicus DELTAH glycerol-1-phosphate + NAD(P)+
-
?
sn-glycerol-1-phosphate + NAD+
-
Methanothermobacter thermautotrophicus dihydroxyacetone phosphate + NADH
-
r
sn-glycerol-1-phosphate + NAD+
-
Methanothermobacter thermautotrophicus DELTAH dihydroxyacetone phosphate + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Methanothermobacter thermautotrophicus