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Literature summary for 1.1.1.248 extracted from

  • Ziegler, J.; Voigtlaender, S.; Schmidt, J.; Kramell, R.; Miersch, O.; Ammer, C.; Gesell, A.; Kutchan, T.M.
    Comparative transcript and alkaloid profiling in Papaver species identifies a short chain dehydrogenase/reductase involved in morphine biosynthesis (2006), Plant J., 48, 177-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene salR, DNA and amino acid sequence determination and analysis, expression profile, overexpression in Escherichia coli strain SG13009 as His6-tagged enzyme Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, recombinant enzyme Papaver somniferum
0.023
-
DNA with apurinic site pH 9.5, 30°C, recombinant enzyme, oxidation reaction Papaver somniferum
0.031
-
salutaridine pH 6.0, 30°C, recombinant enzyme, reduction reaction Papaver somniferum
0.08
-
NADPH pH 6.0, 30°C, recombinant enzyme, reduction reaction Papaver somniferum
0.198
-
NADP+ pH 9.5, 30°C, recombinant enzyme, oxidation reaction Papaver somniferum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34100
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE Papaver somniferum
40000
-
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE Papaver somniferum
47100 50100 recombinant enzyme, gel filtration Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Papaver somniferum alkaloid composition of the organism ?
-
?
additional information Papaver somniferum salR alkaloid composition of the organism ?
-
?
salutaridine + NADPH + H+ Papaver somniferum a step in the unique biosynthesis of morphine via codeine, overview (7S)-salutaridinol + NADP+
-
r
salutaridine + NADPH + H+ Papaver somniferum salR a step in the unique biosynthesis of morphine via codeine, overview (7S)-salutaridinol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Papaver somniferum Q071N0 gene salR; salR
-
Papaver somniferum salR Q071N0 gene salR; salR
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain SG13009 Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information alkaloid composition of the organism Papaver somniferum ?
-
?
additional information no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol Papaver somniferum ?
-
?
additional information alkaloid composition of the organism Papaver somniferum salR ?
-
?
additional information no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol Papaver somniferum salR ?
-
?
salutaridine + NADPH + H+ a step in the unique biosynthesis of morphine via codeine, overview Papaver somniferum (7S)-salutaridinol + NADP+
-
r
salutaridine + NADPH + H+ NADPH-dependent reduction of the 7-keto group Papaver somniferum (7S)-salutaridinol + NADP+ no formation of 7-epi-salutaridinol r
salutaridine + NADPH + H+ a step in the unique biosynthesis of morphine via codeine, overview Papaver somniferum salR (7S)-salutaridinol + NADP+
-
r
salutaridine + NADPH + H+ NADPH-dependent reduction of the 7-keto group Papaver somniferum salR (7S)-salutaridinol + NADP+ no formation of 7-epi-salutaridinol r

Subunits

Subunits Comment Organism
monomer 1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE Papaver somniferum

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the family of short-chain dehydrogenases/reductase Papaver somniferum
SalR
-
Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35 recombinant enzyme, reduction reaction Papaver somniferum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 recombinant enzyme, 50% of maximal activity at 20°C and 40°C, reduction reaction Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 recombinant enzyme, reduction reaction Papaver somniferum
9.5
-
oxidation reaction, recombinant enzyme Papaver somniferum

pH Range

pH Minimum pH Maximum Comment Organism
4.7 7.5 sharp increase in activity at pH 4.7, half-maximal activity at pH 7.5, inactive at pH 9.0, recombinant enzyme, reduction reaction Papaver somniferum
9 10 maximal activity at pH 9.5, sharp decline in activity at pH 10.0, oxidation reaction, recombinant enzyme Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
NAD+ 5% of the activity with NADP+, oxidation reaction, recombinant enzyme Papaver somniferum
NADH 20% of the activity with NADPH, reduction reaction, recombinant enzyme Papaver somniferum
NADP+ preferred cofactor, oxidation reaction, recombinant enzyme Papaver somniferum
NADPH preferred cofactor, reduction reaction, recombinant enzyme Papaver somniferum

pI Value

Organism Comment pI Value Maximum pI Value
Papaver somniferum amino acid sequence calculation
-
4.7