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Literature summary for 1.1.1.236 extracted from

  • Nakajima, K.; Hashimoto, T.
    Two tropinone reductases, that catalyze opposite stereospecific reductions in tropane alkaloid biosynthesis, are localized in plant root with different cell-specific patterns (1999), Plant Cell Physiol., 40, 1099-1107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
TRII cDNA, in Escherichia coli expression vector pET21d Datura stramonium
TRII cDNA, in Escherichia coli expression vector pET21d Hyoscyamus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Datura stramonium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
3 * 29000, SDS-PAGE Hyoscyamus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Atropa belladonna
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus niger
-
pseudotropine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Atropa belladonna
-
-
-
Datura stramonium
-
-
-
Hyoscyamus niger
-
-
-
no activity in Nicotiana tabacum
-
tobacco
-

Purification (Commentary)

Purification (Comment) Organism
-
Datura stramonium
-
Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
endodermis
-
Hyoscyamus niger
-
root
-
Datura stramonium
-
root
-
Atropa belladonna
-
root
-
Hyoscyamus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Atropa belladonna pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?

Subunits

Subunits Comment Organism
trimer
-
Datura stramonium
trimer
-
Atropa belladonna
trimer 3 * 29000, SDS-PAGE Hyoscyamus niger