Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue | nucleotide analog, competitive with respect to NADP+, noncompetitive to androsterone | Mesocricetus auratus | |
Hexestrol | hydroxysteroid analog, uncompetitive versus NADP+, competitive versus androsterone | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
5alpha-androstane-3,17-dione | - |
Mesocricetus auratus | |
0.00016 | - |
NADPH | - |
Mesocricetus auratus | |
0.0026 | - |
NADP+ | - |
Mesocricetus auratus | |
0.0094 | - |
3alpha-hydroxy-5alpha-androstan-17-one | - |
Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi mechanism, NADP+ binds to the free enzyme before the hydroxysteroid substrate, the ketosteroid product leaves first, followed by NADPH | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mesocricetus auratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha-hydroxy-5alpha-androstan-17-one + NADP+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADPH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
inactivated in absence of ligand, protected only by the addition of NADP+ | Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | A-specific | Mesocricetus auratus | |
NADPH | pro-R-hydrogen atom is transferred to the carbonyl substrate, A-specific | Mesocricetus auratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
Cibacron blue | slope effect, 0.025 mM androsterone, varying NADP+ concentrations | Mesocricetus auratus | |
0.0002 | - |
Cibacron blue | slope effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
0.0005 | - |
Cibacron blue | intercept effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
0.0029 | - |
Hexestrol | slope effect, 0.25 mM NADP+, varying androsterone concentrations | Mesocricetus auratus | |
0.0031 | - |
Hexestrol | intercept effect, 0.025 mM androsterone, varying NADP+ concentrations | Mesocricetus auratus |