Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with 6-[(5-chloro-3-methyl-1-benzofuran-2-yl)sulfonyl]pyridazin-3(2H)-one, 20 mg/ml protein in 50 mM diammonium hydrogen citrate, pH 5.0, mixed with NADP+-containing solution in a ratio 1:3, hanging drop vapour diffusion method, soaking in 6-[(5-chloro-3-methyl-1-benzofuran-2-yl)sulfonyl]pyridazin-3(2H)-one solution, containing 5% v/v isopropanol, 5% v/v DMSO, 5% w/v beta-cyclodextrin, 25% w/v PEG 6000, 50 mM diammonium hydrogen citrate pH 5.0, and saturated pyridazinone-type inhibitor, 20°C, 3 days, X-ray diffraction structure determination and analysis at 0.95-1.43 A resolution, modeling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-[(5-chloro-3-methyl-1-benzofuran-2-yl)sulfonyl]pyridazin-3(2H)-one | binding structure, inhibition mechanism, residues Trp111, His110, Thr113, Tyr48, Trp20, and Phe122, and the residues of the C-terminal loop Ala299, Leu300, Leu301, Ser302, Cys303 are involved, overview | Homo sapiens | |
fidarestat | complex formation with the enzyme | Homo sapiens | |
IDD 594 | binding structure, inhibition mechanism, overview | Homo sapiens | |
minalrestat | complex formation with the enzyme | Homo sapiens | |
sorbinil | complex formation with the enzyme | Homo sapiens | |
Tolrestat | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, ALR2 is a TIM-barrel-shpaped aldo-keto reductase with a C-terminal loop, residues Ala299, Leu300, Leu301, Ser302, Cys303, involved in substrate and inhibitor binding | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldose + NADPH + H+ | Homo sapiens | first and rate-limiting step in the polyol pathway | alditol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15121 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alditol + NAD(P)+ = aldose + NAD(P)H + H+ | reaction mechanism, catalytic residues are Tyr48, Lys77, and His110, C-terminal loop residues Ala299, Leu300, Leu301, Ser302, Cys303 are involved in substrate and inhibitor binding | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldose + NADPH + H+ | - |
Homo sapiens | alditol + NADP+ | - |
? | |
aldose + NADPH + H+ | first and rate-limiting step in the polyol pathway | Homo sapiens | alditol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, ALR2 is a TIM-barrel-shpaped aldo-keto reductase with a C-terminal loop, residues Ala299, Leu300, Leu301, Ser302, Cys303, involved in substrate and inhibitor binding | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aldose reductase | - |
Homo sapiens |
ALR2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |