Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | CaMNR1 is induced in leaves by bacterial infection and abiotic elicitor treatments. Under drought conditions, the CaMNR1 transcript is rapidly induced within 1 h and thereafter gradually increased to 25 h, while H2O2, cold and mechanical wounding treatment do not induce CaMNR1 expression in pepper leaves | Capsicum annuum |
Cloned (Comment) | Organism |
---|---|
gene AtSDR1, overexpression in Escherichia coli | Arabidopsis thaliana |
gene CaMNR1, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression in Escherichia coli and in transgenic Arabidopsis thaliana plants | Capsicum annuum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | AtSDR1 virus-induced gene silencing and ectopic overexpression in Arabidopsis thaliana plants leads to significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and lower expression levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, phenotype, overview | Arabidopsis thaliana |
additional information | CaMNR1 tobacco rattle virus-mediated virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis thaliana plants, transgenic CaMNR1-silenced pepper plants are significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and express lower levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, while CaMNR1-overexpression transgenic Arabidopsis thaliana plants exhibit enhanced resistance to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato DC3000 and the biotrophic pathogen Hyaloperonospora parasitica isolate Noco2, accompanied by induction of AtPR1 and AtPDF1.2, phenotypes, overview | Capsicum annuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Capsicum annuum |
34700 | - |
1 * 34700, about, sequence calculation | Capsicum annuum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-menthone + NADPH + H+ | Arabidopsis thaliana | - |
(+)-neomenthol + NADP+ | - |
r | |
(-)-menthone + NADPH + H+ | Capsicum annuum | in vitro antimicrobial activities of menthone, neomenthol and menthol, overview | (3S)-neomenthol + NADP+ | - |
r | |
additional information | Capsicum annuum | CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview | ? | - |
? | |
additional information | Arabidopsis thaliana | the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M2E2 | pathogen responsive gene AtSDR1 | - |
Capsicum annuum | B2X050 | pathogen responsive gene CaMNR1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Capsicum annuum |
recombinant enzyme from Escherichia coli to homogeneity | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Capsicum annuum | - |
fruit | in red, not in green, fruits | Capsicum annuum | - |
leaf | the enzyme is induced in pepper leaves during compatible and incompatible interactions with Xcv, and by bacterial infection and abiotic elicitor treatments | Capsicum annuum | - |
additional information | CaMNR1 is expressed in an organ-specific manner, no activity in uninduced leaves, stems, roots, or green fruits | Capsicum annuum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-menthone + NADPH + H+ | - |
Arabidopsis thaliana | (+)-neomenthol + NADP+ | - |
r | |
(-)-menthone + NADPH + H+ | in vitro antimicrobial activities of menthone, neomenthol and menthol, overview | Capsicum annuum | (3S)-neomenthol + NADP+ | - |
r | |
(-)-menthone + NADPH + H+ | CaMNR1 converts (-)-menthone to 93% (3S)-neomenthol and 7% (3R)-menthol at pH 7.5, and to 72% (3S)-neomenthol and 28% (3R)-menthol at pH 9.0 with NADPH as a cofactor | Capsicum annuum | (3S)-neomenthol + NADP+ | - |
r | |
additional information | CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview | Capsicum annuum | ? | - |
? | |
additional information | the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34700, about, sequence calculation | Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
menthone:(+)-(3S)-neomenthol reductase | - |
Capsicum annuum |
menthone:(+)-(3S)-neomenthol reductase | - |
Arabidopsis thaliana |
More | the enzyme is a member of the short chain dehydrogenase/reductase, SDR, superfamily | Arabidopsis thaliana |
More | the enzyme is a member of the short chain dehydrogenase/reductase, SDR, superfamily, which adopts a unique alpha/beta structure known as the Rossmann fold | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pH alters the stereospecificity of the enzyme | Capsicum annuum |
7.5 | - |
reduction reaction | Capsicum annuum |
9 | - |
oxidation reaction | Capsicum annuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Capsicum annuum | |
NADPH | the enzyme contains the coenzyme binding domain motif I: GxxxGxG | Capsicum annuum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Capsicum annuum | sequence calculation | - |
5.39 |