Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful, together with menthone:(+)-neomenthol reductase (EC 1.1.1.208), for production of (1R,2S,5R)-(-)-menthol and (1S,2S,5R)-(+)-neomenthol from pulegone, development of a one-pot synthesis method, overview. Menthol isomers, (1R,2S,5R)-(-)-menthol, (1R,2S,5S)-(+)-isomenthol, (1S,2S,5R)-(+)-neomenthol, and (1R,2R,5R)-(+)-neoisomenthol, and carvone are used as additives in oral hygiene products and flavors in food and beverages, or cosmetics | Mentha x piperita |
Cloned (Comment) | Organism |
---|---|
recombinant expression of codon-optimized, C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, recombinant expression of three multigene constructs: (i) NtDBR-His6-SD-MMRHis6-SD-His6-MNMR (DMN), (ii) NtDBR-His6-SD-MMRHis6 (DM), and (iii) NtDBR-His6-SD-His6-MNMR in Escherichia coli strain NiCO2(DE3), optimization of multigene expression | Mentha x piperita |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of a one-pot (bio)synthesis of (1R,2S,5R)-(-)-menthol and (1S,2S,5R)-(+)-neomenthol from pulegone, using recombinant Escherichia coli extracts that contain the biosynthetic genes for an ene-reductase NtDBR from Nicotiana tabacum and two menthone dehydrogenases, MMR and MNMR from Mentha piperita, a modular engineering strategy to improve the final production level. Multigene-construct-derived enzyme mixtures production of menthone, isomenthone, menthol, and neomenthol, but no or poor production of neoisomenthol and isomenthol, overview | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-menthone + NADPH + H+ | Mentha x piperita | menthone reduction is the physiological reaction of enzyme MMR | (-)-menthol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | Q5CAF4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain NiCO2(DE3) by nickel affinity chromatography and gel filtration | Mentha x piperita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-menthone + NADPH + H+ | - |
Mentha x piperita | (-)-menthol + NADP+ | - |
r | |
(-)-menthone + NADPH + H+ | menthone reduction is the physiological reaction of enzyme MMR | Mentha x piperita | (-)-menthol + NADP+ | - |
r | |
(2R,5R)-isomenthone + NADPH + H+ | - |
Mentha x piperita | (-)-menthol + NADP+ | - |
r | |
(2S,5R)-menthone + NADPH + H+ | - |
Mentha x piperita | (-)-menthol + NADP+ | - |
r | |
additional information | enzyme MMR has a preference for menthol over neomenthol from menthone and neoisomenthol over isomenthol with isomenthone | Mentha x piperita | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
menthone:(-)-menthol reductase | - |
Mentha x piperita |
MMR | - |
Mentha x piperita |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mentha x piperita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.63 | - |
(2S,5R)-menthone | recombinant His6-tagged enzyme, pH 7.0, 30°C | Mentha x piperita | |
2 | - |
(2R,5R)-isomenthone | recombinant His6-tagged enzyme, pH 7.0, 30°C | Mentha x piperita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mentha x piperita | |
NADPH | - |
Mentha x piperita |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in menthol biosynthesis in the peppermint pathway, overview | Mentha x piperita |