Cloned (Comment) | Organism |
---|---|
gene TR1, enzyme overexpression via leaves transformation with Agrobacterium tumefaciens AGL0, quantitative PCR enzyme expression analysis | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme suppression in potato plants by RNA interference (RNAi) and enzyme overexpression. The overexpression and gene knockdown of StTRI alters tropinone reduction in vivo, but has no influence on calystegine accumulation,in contrast to genetic manipulation of isozyme St-TRII, EC 1.1.1.236, phenotypes, calystegine analysis overview. Higher availability of tropinone, when StTRI gene expression is reduced, and enhanced formation of pseudotropine, that may result in a higher calystegine accumulation, are not evident | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum tuberosum | tropinone feeding experiments | ? | - |
? | |
tropinone + NADPH + H+ | Solanum tuberosum | Solanum tuberosum tropinone reductase I reduces tropinone in vivo | tropine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | Q9AR59 | cv. Desiree | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | tropinone feeding experiments | Solanum tuberosum | ? | - |
? | |
tropinone + NADPH + H+ | - |
Solanum tuberosum | tropine + NADP+ | - |
r | |
tropinone + NADPH + H+ | Solanum tuberosum tropinone reductase I reduces tropinone in vivo | Solanum tuberosum | tropine + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TR1 | - |
Solanum tuberosum |
TRI | - |
Solanum tuberosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Solanum tuberosum | |
NADPH | - |
Solanum tuberosum |
General Information | Comment | Organism |
---|---|---|
physiological function | tropine forming tropinone reductase (TRI) catalyzes a tropinone reduction competing with TRII, EC 1.1.1.236. Tropine formation is essential in the course of the biosynthesis of the medicinal tropine alkaloids atropine and scopolamine that are not found in potato | Solanum tuberosum |