Cloned (Comment) | Organism |
---|---|
gene trI, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Solanum dulcamara |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Solanum dulcamara | the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview | tropine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum dulcamara | - |
gene trI | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Solanum dulcamara |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | - |
Solanum dulcamara | tropine + NADP+ | - |
r | |
tropinone + NADPH + H+ | the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview | Solanum dulcamara | tropine + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the family of short chain dehydrogenases | Solanum dulcamara |
TRI | - |
Solanum dulcamara |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Solanum dulcamara | |
NADPH | - |
Solanum dulcamara |