Cloned (Comment) | Organism |
---|---|
gene TR1, expression in Atropa belladonna root cultures, derived from transfected leaves using the transformation system via Agrobacterium rhizogenes strain 15834 | Datura stramonium |
wild-type and recombinant enzymes from Atropa belladonna root cultures by ammonium sulfate fractionation | Atropa belladonna |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of tropinone reductases TRI and TRII, EC 1.1.1.236, from Datura stramonium alters alkaloid composition in Atropa belladonna root cultures, overview | Atropa belladonna |
additional information | overexpression of tropinone reductases TRI and TRII, EC 1.1.1.236, from Datura stramonium alters alkaloid composition in Atropa belladonna root cultures, overview | Datura stramonium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Atropa belladonna | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Datura stramonium | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | tropine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atropa belladonna | - |
- |
- |
Datura stramonium | P50162 | TRI; gene TR1 | - |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant enzymes from Atropa belladonna root cultures by ammonium sulfate fractionation | Datura stramonium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | tissue culture | Atropa belladonna | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
alkaloid spectrum in wild-type and transformed root cultures, overview | Atropa belladonna |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | - |
Atropa belladonna | tropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Datura stramonium | tropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Atropa belladonna | tropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | tropine is the precursor of the tropane alkaloids hyoscyamine and scopolamine | Datura stramonium | tropine + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRI | - |
Atropa belladonna |
TRI | - |
Datura stramonium |
tropine-forming tropinone reductase | - |
Atropa belladonna |
tropine-forming tropinone reductase | - |
Datura stramonium |
tropinone reductase | - |
Atropa belladonna |
tropinone reductase | - |
Datura stramonium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Atropa belladonna |
30 | - |
assay at | Datura stramonium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Atropa belladonna |
7 | - |
assay at | Datura stramonium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Atropa belladonna | |
NADPH | - |
Datura stramonium |