Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | allosteric activator of the enzyme | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a major therapeutic target | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged type II IMPDH in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mycophenolic acid | uncompetitive inhibition, the interaction with the enzyme, inducing striking conformational changes and aggregation, is regulated by GTP, GTP reverses the enzyme aggregation by inhibitor mycophenolic acid, the inhibitor is part of an immunosuppressive drug | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is rate-limiting in the de novo synthesis of guanine nucleotides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type II IMPDH isozyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme from MCF7 cells partially by gel filtration, recombinant His-tagged type II IMPDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CEM cell | HGPRT-deficient acute lymphoblastic leukemia cell line | Homo sapiens | - |
K6H6/B5 cell | HGPRT-deficient hybridoma cell line | Homo sapiens | - |
MCF7 cell | breast cancer cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is rate-limiting in the de novo synthesis of guanine nucleotides | Homo sapiens | ? | - |
? | |
additional information | the enzyme interacts with GTP which leads to formation of linear arrays, GTP reverses the enzyme aggregation by inhibitor mycophenolic acid | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Homo sapiens |
inosine monophosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |