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Literature summary for 1.1.1.2 extracted from

  • Ying, X.; Wang, Y.; Badiei, H.R.; Karanassios, V.; Ma, K.
    Purification and characterization of an iron-containing alcohol dehydrogenase in extremely thermophilic bacterium Thermotoga hypogea (2007), Arch. Microbiol., 187, 499-510.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADPH pH 8.0, 80°C Pseudothermotoga hypogea
0.02
-
NADP+ pH 11.0, 80°C Pseudothermotoga hypogea
0.45
-
Butyraldehyde pH 8.0, 80°C Pseudothermotoga hypogea
1.9
-
butanol pH 11.0, 80°C Pseudothermotoga hypogea
3.1
-
acetaldehyde pH 8.0, 80°C Pseudothermotoga hypogea
9.7
-
ethanol pH 11.0, 80°C Pseudothermotoga hypogea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron a fully active enzyme contains iron of 1.02 gatoms/subunit Pseudothermotoga hypogea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Pseudothermotoga hypogea
70000
-
gel filtration Pseudothermotoga hypogea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aldehyde + NADPH + H+ Pseudothermotoga hypogea the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols alcohol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pseudothermotoga hypogea
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is oxygen sensitive. Loss of enzyme activity by exposure to oxygen could be recovered by incubation with dithiothreitol and Fe2+ Pseudothermotoga hypogea

Purification (Commentary)

Purification (Comment) Organism
-
Pseudothermotoga hypogea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57
-
-
Pseudothermotoga hypogea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + NADP+
-
Pseudothermotoga hypogea ?
-
?
1,4-butanediol + NADP+
-
Pseudothermotoga hypogea ?
-
?
1,5-pentanediol + NADP+
-
Pseudothermotoga hypogea ?
-
?
1-butanol + NADP+
-
Pseudothermotoga hypogea butanal + NADPH + H+
-
?
1-butanol + NADP+
-
Pseudothermotoga hypogea butyraldehyde + NADPH + H+
-
r
1-heptanol + NADP+
-
Pseudothermotoga hypogea heptanal + NADPH + H+
-
?
1-octanol + NADP+
-
Pseudothermotoga hypogea octanal + NADPH + H+
-
?
1-pentanol + NADP+
-
Pseudothermotoga hypogea n-pentanal + NADPH + H+
-
?
1-propanol + NADP+
-
Pseudothermotoga hypogea ?
-
?
2-phenylethanol + NADP+
-
Pseudothermotoga hypogea phenylacetaldehyde + NADPH + H+
-
?
aldehyde + NADPH + H+ the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols Pseudothermotoga hypogea alcohol + NADP+
-
?
ethanol + NADP+
-
Pseudothermotoga hypogea acetaldehyde + NADPH + H+
-
r
hexyl alcohol + NADP+
-
Pseudothermotoga hypogea hexanal + NADPH + H+
-
?
methylglyoxal + NADPH
-
Pseudothermotoga hypogea ?
-
?
additional information no activity with methanol, 2-propanol, 2-butanol and acetone Pseudothermotoga hypogea ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Pseudothermotoga hypogea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
activity increases along with the rise of temperature up to 95°C Pseudothermotoga hypogea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life: 10 h Pseudothermotoga hypogea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
ethanol pH 11.0, 80°C Pseudothermotoga hypogea
15
-
acetaldehyde pH 8.0, 80°C Pseudothermotoga hypogea
48
-
butanol pH 11.0, 80°C Pseudothermotoga hypogea
48
-
Butyraldehyde pH 8.0, 80°C Pseudothermotoga hypogea
50
-
NADP+ pH 11.0, 80°C Pseudothermotoga hypogea
52
-
NADPH pH 8.0, 80°C Pseudothermotoga hypogea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
production of alcohol Pseudothermotoga hypogea
11
-
oxidation of alcohol Pseudothermotoga hypogea