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Literature summary for 1.1.1.194 extracted from

  • Alarcon, B.; de Armas, R.; Vicente, C.; Legaz, M.
    Inhibition by substrates of a coniferyl alcohol dehydrogenase purified from sugarcane stalks (2019), Curr. Enzyme Inhib., 15, 206-214 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
coniferyl alcohol the enzyme is inhibited by an excess of coniferyl alcohol from 0.017 mM Saccharum officinarum
sinapyl alcohol
-
Saccharum officinarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00178
-
sinapyl alcohol at pH 7.9 and 30°C Saccharum officinarum
0.00303
-
coniferyl alcohol at pH 7.9 and 30°C Saccharum officinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl alcohol + NADP+ Saccharum officinarum
-
coniferyl aldehyde + NADPH + H+
-
r
coniferyl aldehyde + NADPH + H+ Saccharum officinarum
-
coniferyl alcohol + NADP+
-
r
sinapyl alcohol + NADP+ Saccharum officinarum
-
sinapyl aldehyde + NADPH + H+
-
r
sinapyl aldehyde + NADPH + H+ Saccharum officinarum
-
sinapyl alcohol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Saccharum officinarum
-
sugarcane, cultivar My 55-14
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography and Sephadex G-100 gel filtration Saccharum officinarum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Saccharum officinarum
-
stem
-
Saccharum officinarum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
cell-free extract, at pH 7.9 and 30°C Saccharum officinarum
36.21
-
124.8fold purified enzyme, at pH 7.9 and 30°C Saccharum officinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl alcohol + NADP+
-
Saccharum officinarum coniferyl aldehyde + NADPH + H+
-
r
coniferyl aldehyde + NADPH + H+
-
Saccharum officinarum coniferyl alcohol + NADP+
-
r
sinapyl alcohol + NADP+
-
Saccharum officinarum sinapyl aldehyde + NADPH + H+
-
r
sinapyl aldehyde + NADPH + H+
-
Saccharum officinarum sinapyl alcohol + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Saccharum officinarum

Synonyms

Synonyms Comment Organism
CAD
-
Saccharum officinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 22
-
Saccharum officinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Saccharum officinarum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Saccharum officinarum
NADPH
-
Saccharum officinarum