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Literature summary for 1.1.1.191 extracted from

  • Ludwig-Muller, J.; Schramm, P.; Hilgenberg, W.
    Indole-3-acetaldehyde reductase in Phycomyces blakesleeanus. Characterization of the enzyme (1990), Physiol. Plant., 80, 472-478.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
hydroxymethylindole 42% activation at 0.2 mM Phycomyces blakesleeanus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 59% inhibition at 1 mM Phycomyces blakesleeanus
Hg2+ 50% inhibition at 0.01 mM Phycomyces blakesleeanus
Indole-3-aldehyde slight inhibition at 0.2 mM Phycomyces blakesleeanus
indole-3-glycolate 62% inhibition at 0.2 mM Phycomyces blakesleeanus
KI 23% inhibition at 1 mM Phycomyces blakesleeanus
Mn2+ 46% inhibition at 1 mM Phycomyces blakesleeanus
NADP+ non-competitive inhibition, half-maximal inhibition at 0.2 mM Phycomyces blakesleeanus
Zn2+ 36% inhibition at 1 mM Phycomyces blakesleeanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADPH
-
Phycomyces blakesleeanus
0.2
-
Indole-3-acetaldehyde
-
Phycomyces blakesleeanus
0.645
-
dihydroxyacetone phosphate
-
Phycomyces blakesleeanus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 22% activation at 0.1 mM Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indole-3-acetaldehyde + NADPH Phycomyces blakesleeanus
-
indole-3-ethanol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Phycomyces blakesleeanus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture maximum enzyme activity from cultures after 48 h Phycomyces blakesleeanus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
light-grown cells Phycomyces blakesleeanus
0.11
-
dark-grown cells Phycomyces blakesleeanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADPH + H+ very low activity Phycomyces blakesleeanus ethanol + NADP+
-
?
benzaldehyde + NADPH 2.5fold higher activity than for indole-3-acetaldehyde Phycomyces blakesleeanus benzyl alcohol + NADP+
-
?
dihydroxyacetonephosphate + NADPH well accepted substrate Phycomyces blakesleeanus glycerol 1-phosphate + NADP+
-
?
indole-3-acetaldehyde + NADPH
-
Phycomyces blakesleeanus indole-3-ethanol + NADP+
-
?
indole-3-aldehyde + NADPH 19% of the activity of indole-3-acetaldehyde Phycomyces blakesleeanus indole-3-ol + NADP+
-
?
phenylacetaldehyde + NADPH + H+ well accepted substrate Phycomyces blakesleeanus phenylethanol + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Phycomyces blakesleeanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
Phycomyces blakesleeanus

Cofactor

Cofactor Comment Organism Structure
NADP+ with NAD+ 10times lower activity Phycomyces blakesleeanus