Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | dependent on | Ovis aries | |
additional information | 4fold activation by heat treatment | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
Y55F | complete elimination of PGD2, and PQ reductase activities, 27fold stimulation of PGH2 reductase activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
9,10-phenanthrenequinone | competitive inhibition of the PGD2 11-ketoreductase activity, not of PGH2 9-,11-endoperoxide reductase activity | Rattus norvegicus | |
additional information | no inhibition by 9,10-phenanthrenequinone | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
cytosol | - |
Trypanosoma brucei | 5829 | - |
membrane | associated | Ovis aries | 16020 | - |
microsome | - |
Ovis aries | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 16000 | Ovis aries |
17000 | - |
- |
Ovis aries |
33000 | - |
- |
Trypanosoma brucei |
36666 | - |
1 * 36666, lung enzyme, sequence calculation | Rattus norvegicus |
36670 | - |
lung enzyme, sequence calculation | Rattus norvegicus |
37000 | - |
lung and liver isozymes | Bos taurus |
37000 | - |
lung isozyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin D2 + NADPH + H+ | Homo sapiens | - |
prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | Rattus norvegicus | - |
prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | Bos taurus | - |
prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | Oryctolagus cuniculus | - |
prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin H2 + NADPH + H+ | Homo sapiens | - |
prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Rattus norvegicus | - |
prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Bos taurus | - |
prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Oryctolagus cuniculus | - |
prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Ovis aries | - |
prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Trypanosoma brucei | - |
prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | Bos taurus | enzyme also shows PGH2 9-,11-endoperoxide reductase activity | prostaglandin F2alpha + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
to isozymes in lung and liver | - |
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
two isozymes in lung and liver | - |
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme from lung, to homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ | Tyr55 is critical for the reaction, the reactions with different substrates might follow different mechanisms | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | liver-type isozyme | Oryctolagus cuniculus | - |
lung | - |
Rattus norvegicus | - |
lung | - |
Bos taurus | - |
lung | lung-type isozyme | Homo sapiens | - |
lung | PGH2 9-,11-endoperoxide reductase activity lung-type isozyme | Bos taurus | - |
seminal vesicle | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH | i.e. PQ | Homo sapiens | ? | - |
? | |
9,10-phenanthrenequinone + NADPH | i.e. PQ | Rattus norvegicus | ? | - |
? | |
9,10-phenanthrenequinone + NADPH | i.e. PQ | Bos taurus | ? | - |
? | |
9,10-phenanthrenequinone + NADPH | i.e. PQ | Oryctolagus cuniculus | ? | - |
? | |
additional information | enzyme does not reduce prostaglandins D2 and E2 | Trypanosoma brucei | ? | - |
? | |
additional information | enzyme shows no prostaglandin D2 11-ketoreductase activity, and no glutathione S-transferase activity, although it is similar to the glutathione S-transferase | Ovis aries | ? | - |
? | |
prostaglandin D2 + NADPH + H+ | - |
Homo sapiens | prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | - |
Rattus norvegicus | prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | - |
Bos taurus | prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | - |
Oryctolagus cuniculus | prostaglandin 9alpha,11beta-F2 + NADP+ | product is involved in bronchial, vascular, and arterial smooth muscle contraction, product inhibits platelet aggregation and activates urinary excretion | ? | |
prostaglandin D2 + NADPH + H+ | - |
Homo sapiens | prostaglandin 9alpha,11beta-F2 + NADP+ | i.e. 11-epi PGF2alpha | ? | |
prostaglandin D2 + NADPH + H+ | - |
Rattus norvegicus | prostaglandin 9alpha,11beta-F2 + NADP+ | i.e. 11-epi PGF2alpha | ? | |
prostaglandin D2 + NADPH + H+ | - |
Bos taurus | prostaglandin 9alpha,11beta-F2 + NADP+ | i.e. 11-epi PGF2alpha | ? | |
prostaglandin D2 + NADPH + H+ | - |
Oryctolagus cuniculus | prostaglandin 9alpha,11beta-F2 + NADP+ | i.e. 11-epi PGF2alpha | ? | |
prostaglandin H2 + NADPH + H+ | - |
Homo sapiens | prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Rattus norvegicus | prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Bos taurus | prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Oryctolagus cuniculus | prostaglandin D2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Ovis aries | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Trypanosoma brucei | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Trypanosoma brucei | prostaglandin F2alpha + NADP+ | no stereochemic determination/identification of the product | ? | |
prostaglandin H2 + NADPH + H+ | enzyme also shows PGH2 9-,11-endoperoxide reductase activity | Homo sapiens | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | enzyme also shows PGH2 9-,11-endoperoxide reductase activity | Rattus norvegicus | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | enzyme also shows PGH2 9-,11-endoperoxide reductase activity | Bos taurus | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | enzyme also shows PGH2 9-,11-endoperoxide reductase activity | Oryctolagus cuniculus | prostaglandin F2alpha + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16000 | Ovis aries |
monomer | 1 * 36666, lung enzyme, sequence calculation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the AKR aldo-keto reductase superfamily | Trypanosoma brucei |
More | enzyme belongs to the AKR aldo-keto-reductase superfamily | Homo sapiens |
More | enzyme belongs to the AKR aldo-keto-reductase superfamily | Rattus norvegicus |
More | enzyme belongs to the AKR aldo-keto-reductase superfamily | Bos taurus |
More | enzyme belongs to the AKR aldo-keto-reductase superfamily | Oryctolagus cuniculus |
PG endoperoxide reductase | - |
Ovis aries |
PGD 11-ketoreductase | - |
Homo sapiens |
PGD 11-ketoreductase | - |
Rattus norvegicus |
PGD 11-ketoreductase | - |
Bos taurus |
PGD 11-ketoreductase | - |
Oryctolagus cuniculus |
PGD2 11-ketoreductase | - |
Homo sapiens |
PGD2 11-ketoreductase | - |
Rattus norvegicus |
PGD2 11-ketoreductase | - |
Bos taurus |
PGD2 11-ketoreductase | - |
Oryctolagus cuniculus |
PGF synthase | - |
Homo sapiens |
PGF synthase | - |
Rattus norvegicus |
PGF synthase | - |
Bos taurus |
PGF synthase | - |
Oryctolagus cuniculus |
PGF2alpha synthase | - |
Trypanosoma brucei |
PGH 9-,11-endoperoxide reductase | - |
Ovis aries |
PGH 9-,11-endoperoxide reductase | - |
Trypanosoma brucei |
PGH2 9-,11-endoperoxide reductase | - |
Bos taurus |
PGH2 9-,11-endoperoxide reductase | - |
Ovis aries |
PGH2 9-,11-endoperoxide reductase | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Rattus norvegicus | |
NADPH | dependent on | Bos taurus | |
NADPH | dependent on | Oryctolagus cuniculus | |
NADPH | dependent on | Ovis aries | |
NADPH | dependent on | Trypanosoma brucei |