Application | Comment | Organism |
---|---|---|
pharmacology | enzyme is a target for cyclooxygenase-independent antineoplastic actions of nonsteroidal anti-inflammatory drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
16 mg/ml purified enzyme complexed with inhibitors acetate, flufenamic acid or indomethacin, hanging drop vapour diffusion method in 0.006 ml drops, 1:1 mixture of protein solution containing 10 mM potassium phosphate, pH 7.0, 1 mM DTT, 1 mM EDTA, 2 mM NADP+, with reservoir solution containing 25% w/v PEG 4000, 100 mM sodium citrate, pH 6.0, 2.5% v/v 2-methyl-2,4-pentanediol, and 800 mM ammonium acetate, 6 days to maximum size, X-ray diffraction structure determination of the complexes and analysis at 1.2-2.1 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | occupies the oxyanion hole of the active site, 2 complex structures | Homo sapiens | |
Flufenamic acid | nonsteroidal anti-inflammatory drug, binds to both the active site and the beta-hairpin loop at the opposite end of the central beta-barrel, complex structure | Homo sapiens | |
indomethacin | nonsteroidal anti-inflammatory drug, 1 molecule binds to the active site, complex structure | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of 3alpha-hydroxysteroid dehydrogenase type II activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42330 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ | active site structure, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acive site structure, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1C3 | - |
Homo sapiens |
More | enzyme belongs to the aldo-keto reductase AKR family, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD | Homo sapiens |
PGFS | - |
Homo sapiens |
prostaglandin F synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki values for the nonsteroidal anti-inflammatory drug inhibitors with 3alpha-androstanediol as a substrate | Homo sapiens |