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Literature summary for 1.1.1.184 extracted from

  • Wang, X.; Lin, C.; Zhang, X.; Liu, Z.; Zheng, Y.
    Improvement of a newly cloned carbonyl reductase and its application to biosynthesize chiral intermediate of duloxetine (2018), Process Biochem., 70, 124-128 .
No PubMed abstract available
Search for more literature for 1.1.1.184

Protein Variants

Protein Variants Comment Organism
A89N/F154Y Vmax and kcat values of the mutant enzyme are enhanced by 2.08 and 3.86fold, respectively, while the Km value decreases by 2.36fold compared to the wild type enzyme Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethyl-3-keto-3-(2-thienyl)-1-keto propanamine + NADPH + H+ Rhodotorula toruloides mutant enzyme A89N/F154Y with yield of 91.2% and S-enantiomeric excess of 99.9% (S)-3-(dimethylamino)-1-(2-thienyl)-1-propanol + NADP+
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Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethyl-3-keto-3-(2-thienyl)-1-keto propanamine + NADPH + H+ mutant enzyme A89N/F154Y with yield of 91.2% and S-enantiomeric excess of 99.9% Rhodotorula toruloides (S)-3-(dimethylamino)-1-(2-thienyl)-1-propanol + NADP+
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 ?

Synonyms

Synonyms Comment Organism
SCR9
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 Rhodotorula toruloides