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Literature summary for 1.1.1.184 extracted from

  • Slupe, A.; Williams, B.; Larson, C.; Lee, L.M.; Primbs, T.; Bruesch, A.J.; Bjorklund, C.; Warner, D.L.; Peloquin, J.; Shadle, S.E.; Gambliel, H.A.; Cusack, B.J.; Olson, R.D.; Charlier, H.A.
    Reduction of 13-deoxydoxorubicin and daunorubicinol anthraquinones by human carbonyl reductase (2005), Cardiovasc. Toxicol., 5, 365-376.
    View publication on PubMed
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
rutin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information recombinant enzyme, steady-state kinetics, molecular modeling and substrate docking studies Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
daunorubicin + NADPH + H+ Homo sapiens reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties daunorubicinol + NADP+
-
 ?
doxorubicin + NADPH + H+ Homo sapiens reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties doxorubicinol + NADP+
-
 ?
menadione + NADPH + H+ Homo sapiens reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties ?
-
 ?
mitroxantrone + NADPH + H+ Homo sapiens reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties ?
-
 ?
naphthazarin + NADPH + H+ Homo sapiens reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16152
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ molecular modeling and enzyme-substrate docking studies, reaction mechanism with anthracyclin substrates Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13-deoxydoxorubicin + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens ?
-
 ?
daunorubicin + NADPH + H+ reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties Homo sapiens daunorubicinol + NADP+
-
 ?
daunorubicin + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens daunorubicinol + NADP+
-
 ?
doxorubicin + NADPH + H+ reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties Homo sapiens doxorubicinol + NADP+
-
 ?
doxorubicin + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens doxorubicinol + NADP+
-
 ?
menadione + NADPH + H+ reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties Homo sapiens ?
-
 ?
menadione + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens ?
-
 ?
mitroxantrone + NADPH + H+ reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties Homo sapiens ?
-
 ?
mitroxantrone + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens ?
-
 ?
naphthazarin + NADPH + H+ reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties Homo sapiens ?
-
 ?
naphthazarin + NADPH + H+ an anthracyclin, formation of a 13-hydroxy-anthracyclin Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More molecular modeling and generation of a three-dimensional structural model Homo sapiens

Synonyms

Synonyms Comment Organism
carbonyl reductase 1
-
 Homo sapiens
CBR1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Homo sapiens