Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 cells | Desulfococcus biacutus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | Desulfococcus biacutus | - |
3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | Desulfococcus biacutus KMRActS (DSM 5651) | - |
3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | Desulfococcus biacutus KMRActS DSM 5651 | - |
3-acetoacetyl-CoA + NADH + H+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | Desulfococcus biacutus | - |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | Desulfococcus biacutus KMRActS (DSM 5651) | - |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | Desulfococcus biacutus KMRActS DSM 5651 | - |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-hydroxybutyryl-CoA + NAD+ | Desulfococcus biacutus | - |
3-acetoacetyl-CoA + NADH + H+ | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | Desulfococcus biacutus KMRActS (DSM 5651) | - |
3-acetoacetyl-CoA + NADH + H+ | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | Desulfococcus biacutus KMRActS DSM 5651 | - |
3-acetoacetyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfococcus biacutus | - |
- |
- |
Desulfococcus biacutus KMRActS (DSM 5651) | - |
- |
- |
Desulfococcus biacutus KMRActS DSM 5651 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Desulfococcus biacutus |
Ni-NTA column chromatography, and Superdex 200 gel filtration | Desulfococcus biacutus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetone-grown cell | - |
Desulfococcus biacutus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.1 | - |
reduction of 250 mM acetoacetyl-CoA in the presence of 0.5 mM NADH, at pH 8.0 and 30°C | Desulfococcus biacutus |
23.3 | - |
oxidation of 0.05 mM 3-hydroxybutyryl-CoA in the presence of 5 mM NAD+, at pH 8.0 and 30°C | Desulfococcus biacutus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Desulfococcus biacutus | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Desulfococcus biacutus KMRActS (DSM 5651) | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Desulfococcus biacutus KMRActS DSM 5651 | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | - |
Desulfococcus biacutus | (S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | - |
Desulfococcus biacutus KMRActS (DSM 5651) | (S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | - |
Desulfococcus biacutus KMRActS DSM 5651 | (S)-3-hydroxybutanoyl-CoA + NAD+ | - |
r | |
3-hydroxybutyryl-CoA + NAD+ | - |
Desulfococcus biacutus | 3-acetoacetyl-CoA + NADH + H+ | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | - |
Desulfococcus biacutus KMRActS (DSM 5651) | 3-acetoacetyl-CoA + NADH + H+ | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | - |
Desulfococcus biacutus KMRActS DSM 5651 | 3-acetoacetyl-CoA + NADH + H+ | - |
? | |
additional information | no reaction is observed with NADP+ | Desulfococcus biacutus | ? | - |
- |
|
additional information | no reaction is observed with NADP+ | Desulfococcus biacutus KMRActS (DSM 5651) | ? | - |
- |
|
additional information | no reaction is observed with NADP+ | Desulfococcus biacutus KMRActS DSM 5651 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, calculated from amino acid sequence | Desulfococcus biacutus |
? | x * 31000, His6-tagged enzyme, SDS-PAGE | Desulfococcus biacutus |
Synonyms | Comment | Organism |
---|---|---|
DebiaDRAFT_04571 | - |
Desulfococcus biacutus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Desulfococcus biacutus | |
NAD+ | dependent on | Desulfococcus biacutus |
Organism | Comment | Expression |
---|---|---|
Desulfococcus biacutus | the enzyme expression is strongly induced in acetone-grown cells | up |