Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.05 mM, 80% inhibition | Clostridium kluyveri | |
iodoacetamide | 1 mM, slight inhibition | Clostridium kluyveri | |
N-ethylmaleimide | 1 mM, slight inhibition | Clostridium kluyveri | |
p-chloromercuribenzoate | 1 mM, complete inhibition | Clostridium kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
3-acetoacetyl-CoA | pH 6.5, 25°C | Clostridium kluyveri | |
0.07 | - |
NADPH | pH 6.5, 25°C | Clostridium kluyveri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
8 * 26000, SDS-PAGE | Clostridium kluyveri |
215000 | - |
gel filtration | Clostridium kluyveri |
220000 | - |
equilibrium sedimentation | Clostridium kluyveri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetoacetyl-CoA + NADPH + H+ | Clostridium kluyveri | synthesis of butyrate | (S)-3-hydroxybutanoyl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium kluyveri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
445 | - |
reduction of 3-acetoacetyl-CoA, pH 6.5, 25°C | Clostridium kluyveri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetoacetyl-CoA + NADPH + H+ | synthesis of butyrate | Clostridium kluyveri | (S)-3-hydroxybutanoyl-CoA + NADP+ | - |
? | |
3-acetoacetyl-CoA + NADPH + H+ | the oxidation of (S)-3-hydroxybutanoyl-CoA at pH 9.5 proceeds with 7% of the rate of the 3-acetoacetyl-CoA reduction. At pH 6.5, the reduction of 3-acetoacetyl-CoA is 14times faster than the oxidation of (S)-3-hydroxybutanoyl-CoA.The enzyme does not oxidize 3-hydroxyvaleryl-CoA or 3-hydroxycaproyl-CoA | Clostridium kluyveri | (S)-3-hydroxybutanoyl-CoA + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 26000, SDS-PAGE | Clostridium kluyveri |
Synonyms | Comment | Organism |
---|---|---|
L(+)-3-hydroxybutyryl-CoA dehydrogenase | - |
Clostridium kluyveri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Clostridium kluyveri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
at the pH optimum 6.5 the reduction of 3-acetoacetyl-CoA is 14times faster than the oxidation of (S)-3-hydroxybutanoyl-CoA at its pH optimum 9.5 | Clostridium kluyveri |
9.5 | - |
at the pH optimum 6.5 the reduction of 3-acetoacetyl-CoA is 14times faster than the oxidation of (S)-3-hydroxybutanoyl-CoA at its pH optimum 9.5 | Clostridium kluyveri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5: about 35% of maximal activity, pH 7.5: about 55% of maximal activity, reduction of 3-acetoacetyl-CoA | Clostridium kluyveri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Clostridium kluyveri | |
NADPH | the rate with NADH is below 0.25% of that with NADPH | Clostridium kluyveri |