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Literature summary for 1.1.1.153 extracted from

  • Tang, Y.; Pei, Z.; Liu, L.; Wang, D.; Kong, L.; Liu, S.; Jiang, X.; Gao, Y.; Ma, H.
    Expression and enzyme activity detection of a sepiapterin reductase gene from Musca domestica larva (2017), Appl. Biochem. Biotechnol., 181, 604-612 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Musca domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02348
-
sepiapterin at pH 6.4 and 37°C Musca domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sepiapterin + NADPH + H+ Musca domestica
-
7,8-dihydrobiopterin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Musca domestica A0A172EJV6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Musca domestica

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Musca domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sepiapterin + NADPH + H+
-
Musca domestica 7,8-dihydrobiopterin + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Musca domestica

Synonyms

Synonyms Comment Organism
MDSPI16 protein
-
Musca domestica
SPR
-
Musca domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Musca domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 the enzymatic activity is high under the temperature range of 40 to 70°C Musca domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Musca domestica

pH Range

pH Minimum pH Maximum Comment Organism
3 6 the enzymatic activity is high under the pH range of 3.0 to 6.0 Musca domestica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Musca domestica